5-hydroxymethylcytosine (5hmC) is considered as a novel DNA modification and plays an important role in cancer, stem cells, and developmental diseases. In this study, we demonstrated the existence of RNA 5hmC modification in mouse brain RNA by using a dot blot analysis method. Our data indicated...
The DNA base 5-hydroxymethylcytosine (5hmC) is produced by enzymatic oxidation of 5-methylcytosine (5mC) by 5mC oxidases (the Tet proteins). Since 5hmC is recognized poorly by DNA methyltransferases, DNA methylation may be lost at 5hmC sites during DNA replication. In addition, 5hmC can be oxidi...
Here, we determined the 5-hydroxymethylcytosine (5hmC), 5-methylcytosine (5mC), Ten Eleven Translocation (TETs), and DNA methyltransferases (DNMTs) levels in epithelial and inflammatory cells of labial salivary glands (LSG) from Sjögren's syndrome (SS)-patients and the effect of cytokines o...
Emerging data have demonstrated that 5-methylcytosine (5-mC) and its oxidized products 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-fC), and 5-carboxylcytosine (5-CaC) play unique roles in several biological processes, including the control of gene expression and in the pathogenesis of c...
DNA methylation at the fifth position of cytosines (5mC) represents a major epigenetic modification in mammals. The recent discovery of 5-hydroxymethylcytosine (5hmC), resulting from 5mC oxidation, is redefining our view of the epigenome, as multiple studies indicate that 5hmC is not simply an i...
Herein, a novel and convenient electrogenerated chemiluminescence (ECL) biosensing method for the quantitative detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA was developed by employing thiolated anchor probe DNA to immobilize 5-hydroxymethylcytosine double-stranded DNA (5-hmC-dsDNA)...
5-hydroxymethylcytosine (5hmC) is an epigenetic DNA modification. Tissue-specific reduction in global 5hmC levels has been associated with various types of cancer. One of the challenges associated with detecting 5hmC levels is its extremely low content, especially in blood. The gold-standard for...
Recent reports suggest that the Tet enzyme family catalytically oxidize 5-methylcytosine in mammalian cells. The oxidation of 5-methylcytosine can result in three chemically distinct species – 5-hydroxymethylcytsine, 5-formylcytosine, and 5-carboxycytosine. While the base excision repair machin...
Low temperature coal tar contained a large amount of phenols, aromatic hydrocarbons and alkanes; the separation of phenols from coal tar has a great significance to the deep processing of coal tar. In this work, the separation of m-cresol from cumene and n-heptane by liquid–liquid extraction us...
A series of carbocyclic amines was reacted with 3-aminocrotonitrile to give the 2-arylhydrazone-3-ketiminobutyronitriles 1(a–m). Separately, 2-aminobenzothiazole was treated with hydrazine monohydrate to afford 2-hydrazinobenzothiazole. Then, compounds 1(a–m) were reacted with 2-hydrazinobenzi...
The cyanation of carbonyl compounds with ethyl cyanoformate is catalyzed by 4-dimethylaminopyridine (DMAP) to afford the corresponding cyanohydrin carbonates in excellent yields. The system provides a convenient method for cyanation of carbonyl compounds without using metal catalysts or solvents.
A simple synthetic protocol has been developed involving the three-component reaction between 1,2-diamines, ethyl pyruvate and α-bromo ketones in the presence of FeCl3 as a catalyst. A number of pyrrolo[1,2-a]quinoxaline and pyrrolo[1,2-a]pyrazine derivatives were synthesized in excellent yield...
Chiral secondary alcohols are ubiquitous motifs in numerous natural products, pharmaceuticals, and biological active compounds. Catalytic asymmetric hydrogenation of multi-functionalized ketones provides an effective and powerful synthesis method to construct chiral secondary alcohols. In the as...
The asymmetric transfer hydrogenation (ATH) of ketones catalyzed by Rh-based macrocycles proceeded smoothly in the presence of air with high catalytic activity and enantioselectivity. Even though the S/C ratio (substrate to catalyst molar ratio) was increased up to 2000:1, the ATH of ketone stil...
E- and Z-dialkylvinyloxyboranes, prepared directly from cyclohexyl ethyl ketone, undergo stereo-selective aldol condensations to provide threo- erythro-β-hydroxy-α-methylketones, respectively.
A mannitol derived 2,3-butanediacetal ethyl ketone displays high levels of diastereoselectivity in boron and titanium mediated aldol reactions with a range of aliphatic and aromatic aldehydes to afford syn aldol products in high yield. The stereochemical outcome of the reaction was determined us...
ABSTRACTInexpensive and efficient Cu(I) catalysis is reported for the synthesis of α-hydroxy ketones from propargylic alcohols, CO2, and water via tandem carboxylative cyclization and nucleophilic addition reaction. Notably, hydration of propargylic alcohols can be carried out smoothly under at...
The persistence of synthetic cyclohexyl- and norbornyl-derived ketones was assessed by using OECD 301F and 301D biodegradation tests. While cyclohexyl-derived ketones either reached or came close to the pass level (60%) after 60 d, the corresponding norbornyl derivatives yielded significantly le...
We introduced here a new one-pot, general procedure for the preparation of dialkyl carbonates from alcohols in a straightforward fashion under 1 atm pressure of CO2 using Cs2CO3 and CH2Cl2 as key reagents.
2,3-Bis(dimenthylphosphino)maleic anhydride and also 2,3-bis(dimenthylphosphino)maleimide derivatives have been prepared from 2,3-dichloromaleic anhydride, 2,3-dichloro-N-phenylmaleimide and 2,3-dichloro-N-methylmaleimide, respectively, and dimenthyl(trimethylsilyl)phosphine. These compounds hav...
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