The two title carbon frameworks were synthesized utilizing a new type of iron-induced cyclization reaction of 2-(trimethylsilylmethyl)pentadienal. 2-Methylspiro[4.5]dec-2-en-1-one was obtained from (Z)- and (E)-4-cyclohexylidene-2-(trimethylsilylmethyl)but-2-enal. It was found that the (Z)-subst...

Two APRT− clones (V79-E3 and V79-E1A) were isolated from V79 hamster fibroblasts treated with ethyl methanesulfonate. Selection involved sequential exposure of the mutagenized cells to the adenine analogues 8-azaadenine and 2,6-diaminopurine. To examine the influence of APRT deficiency on cell m...

Publisher SummaryThis chapter reviews the evidence for localized hydrolysis of adenine nucleotides at the synaptic level. A possibility to explain all the data is that at the synaptic level there is an enzyme able to catabolise β,γ-methylene ATP, but the restricted localization of this enzyme ...

a simple and efficient protocol is described for site-specific 15N-labelling of adenine in synthetic oligodeoxynucleotides by post-synthetic substitution with 15N-labelled ammonia of the oxidized methylthio group on purine. A labelled DNA of 20-mer was characterized by NMR spectroscopy.

6-Methylmercaptopurine ribonucleoside-5′-phosphate (MeSPuRMP), the sole metabolite of 6-methylmercaptopurine ribonucleoside (MeSPuRib), is a strong inhibitor of purine de novo synthesis, inducing depletion of intracellular purine nucleotides and subsequent cell death in several tumor cell lines...

The interactions between lima bean lectin (LBL) and adenine were examined using a series of synthetic purine analogs. Binding was sensitive to modification at most positions of the purine ring, suggesting a high degree of specificity for adenine binding. Methylation ofthe 6 NH2-group to MeNH-, M...

In this paper we report the synthesis and characterization of four new nitroaromatic compounds, 2-{6-nitrobenzo[1,3]dioxol-5-(methyleneamino)}benzoic acid (1), 2-{[5-(2-nitrophenyl)furan-2-yl]methylene-amino}benzoic acid (2), 2-{(6-nitrobenzo[1,3]dioxol-5-yl)methylene}hydrazinecarboxamide (3) an...

The reaction of 1,3-cyclohexadione with 2-aminobenzoic acid has produced the 2-(3-oxocyclohex-1-enyl)benzoic acid (HOBz). Subsequent reactions of the ligand with organotin chlorides led to [Me2Sn(OBz)O]2 (1), [Bu2Sn(OBz)O]2 (2), [Ph2Sn(OBz)O]2 (3), [Me3Sn(OBz)] (4), [Bu3Sn(OBz)] (5) and [Ph3Sn(O...

The synthesis of all-carbon quaternary centers remains a challenge. Here we describe studies on the formation of two adjacent all-carbon quaternary centers in the context of the planned synthesis of the bioactive natural products perophoramidine and the communesins. In one approach the key step ...

This study describes a simple and efficient procedure to synthesize a series of benzene-fluorinated 2,2-dimethyl-2,3-dihydro-1H-quinolin-4-ones by the PTSA-catalyzed cyclocondensation reaction of the corresponding ortho-alkynylanilines prepared by the Sonogashira reaction of fluoro-substituted 2...

A novel isoflavone glycoside, 5,4′-dimethoxy-3′-prenylbiochanin 7-O-β-d-galactoside (1), was isolated from the leaves of S. indicus.

We have constructed a version of the chiral three-preon model E6 × SO(10) that satisfies the condition of asymptotic freedom in the metacolor and composite color-flavor sectors. The construction is based on the global color-flavor symmetry group SU(18). By applying the 't Hooft anomaly mat...

Tumbling complementarity is studied in chiral preon models based on the metacolor group E6. By considering the breaking of E6 into each maximal little group in the Higgs phase, several models are found that are in accord with complementarity. Three families of massless fermions (on the metacolor...

A new mechanism is presented for the generation of quark and lepton masses, based on a heavy fourth family and a new sector of massless fermions. The massless fermions have only discrete chiral symmetries and they are confined by the metacolor force. The resulting electroweak corrections may be ...

The degradations of Reactive Brilliant Red X-3B (RBRX-3B) in an electric-assisted microbial system (EAMS), a microbial system (MS) and an electrochemical system (ECS) were compared. The degradation efficiency of RBRX-3B in EAMS (99.8%) was 10.8% higher than the sum in MS (61.9%) and ECS (27.1%) ...

In the present study, we report a comparative study of Reactive Blue 235 (RB235) dye removal using red mud derived biologically synthesized iron nanoparticles (bRMINP) and chemically synthesized iron nanoparticles (cRMINP), both immobilized in barium alginate beads. The parameters like initial R...

Herein, we developed a new CL method for the detection of glucose, exhibiting high sensitivity, low limit of detection, good stability and reliability for analysis of real biological samples. The MOF-235/β-cyclodextrin (β-CD) hybrids were facilely prepared by a simple method, and characterized...

Sensitive and specific monoclonal antibodies (mAbs) against not only human but also mouse, rat, rabbit, dog, cat, bovine, pig, and horse podoplanins (PDPNs) have been established in our previous studies. However, anti-goat PDPN (gPDPN) has not been established yet. PDPN has been utilized as a ly...

A component that causes contraction of the isolated guinea pig tracheal smooth muscle was isolated in homogeneous form from the venom of the pedicellaria of the sea urchin, Toxopneustes pileolus. It is named Contractin A. Contractin A has 18,000 Da with a total residue of 138 amino acids. The mo...

Venomous sea urchins contain various biologically active proteins that are toxic to predators. Contractin A is one such protein contained within the globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus. This protein exhibits several biological activities, such as smooth mus...