In this study, the FAD synthesis pathway was manipulated to increase its cellular concentration and thereby improve the electroactivity of E. coli. In a microbial electrosynthesis (MES) system with neutral red as electron carrier and fumarate as the sole electron acceptor, the engineered strains...

After deleting the gene encoding succinate dehydrogenase, Corynebacterium glutamicum can produce succinate and a considerable amount of acetate and pyruvate as by-products from glucose metabolism, under aerobic conditions. Recently, we identified ynfM in Pantoea ananatis (PaynfM) as a gene encod...

АbstractThe modification of chitosan (Ch) with a surfactant hexadecyl-oligo-oxyethylene hemisuccinate (HOS) as well as properties of modified chitosan succinate (ChS) have been studied. Modification of ChS was made with the use of surfactants of different nature: anionic surfactant - sodium dod...

Methane-utilizing methanotrophs are fascinating systems for methane bioconversion. Methylomonas sp. DH-1, a novel type I methanotroph isolated from brewery sludge, has been evaluated as a promising candidate for an industrial bio-catalyst. Succinate has been considered one of the top building bl...

We studied the effect of YM-39558, orotic acid ethylester, in a focal cerebral ischemia model in anesthetized cats. YM-39558 has good permeability across the blood brain barrier, and in the brain is hydrolyzed to orotic acid, the main active substance. Cats were subjected to permanent occlusion ...

The protective effect of YM-254890, a specific Gαq/11 inhibitor, on laurate-induced peripheral arterial disease in rats was compared with those of prostaglandin E1 (PGE1), beraprost, and clopidogrel. YM-254890 inhibited ADP-induced ex vivo rat platelet aggregation at a dose of 3 μg/kg. Further...

The structure elucidation and biological activity of novel YM-254890 (1) analogues and semi-synthetic derivatives are described. Three natural analogues, YM-254891 (2), YM-254892 (3), and YM-280193 (4), were isolated from the fermentation broth of Chromobacterium sp. QS3666, and two hydrogenated...

Phase separation induced by a two-step temperature jump was studied by time-resolved light scattering and scanning electron microscopy for polystyrene/poly(2-chlorostyrene) blends. A co-continuous domain structure was formed by the first-step jump, with small droplets being formed in these co-co...

Dynamics of phase separation induced by polymerization of 2-chlorostyrene in the presence of polystyrene was studied by an scanning electron microscope and a time-resolved light scattering instrument with varying initial monomer composition θ in the range from 0.4 to 0.6. Phase-separation rate ...

Cloud points of polystyrene/poly(2-chlorostyrene) blends of narrow molecular weight distribution were measured with changing heating rate and molecular weight of polystyrene. Cloud points were also measured for blends containing various amounts of the plasticizer di-n-butyl phthalate. A large he...

The phase behaviour of poly(2-chlorostyrene)/poly(vinyl methyl ether) blends (P2CSPVME) was investigated by means of light scattering and small-angle X-ray scattering (SAXS) using synchrotron radiation. It was found that P2CSPVME blends possess a lower critical solution temperature. Chemically l...

Dielectric relaxation of poly(vinyl methyl ether) (PVME)/poly(2-chlorostyrene) (P2CS) blends with a lower critical solution temperature (LCST) was investigated in the one-phase region over a wide range of temperature. Four distinct dielectrically active processes were observed and identified as ...

In this work, the miscibility of poly(2-chlorostyrene) (P2CS) and poly(vinyl methyl ether) (PVME) mixtures was characterized and compared to polystyrene (PS)/PVME blends. It was found, by light scattering, that P2CSPVME blends exhibit a lower critical solution temperature. Fourier transform infr...

A method for determining the critical micelle concentration (CMC) of various detergents based on fluorescence polarization (anisotropy) of the lipophilic probe 5-dodecanoylaminofluorescein is presented. Nonionic, cationic, anionic, and steroid-based detergents can all be evaluated by this method...

The mechanism and kinetics of the hydrogen abstraction reaction between the NCO radical and CH3OH without and with one water molecule have been investigated theoretically. The potential energy surface profiles have been constructed at the CCSD(T)/6-311++G(2df,2pd)//BH&HLYP/6-311++G(2df,2pd) + ZP...

A novel diamine 4-(4-aminophenoxy)butyl-4-aminophenyl ether (ABAE) was synthesized as a chain extender (CE) by a quick and facile method using microwave reactor. CE was used for the preparation of stimuli-responsive Polyurethane-urea elastomer (PUU) with the ratio of NCO/OH from 2 to 2.5. Synthe...

This paper reviews the role of self-optimizing control (SOC) and necessary conditions of optimality tracking (NCO tracking) as presented by [1]. We show that self-optimizing control is not an alternative to NCO tracking for steady state optimization, but is to be seen as complementary. In self-o...

We investigated whether a polymorphism in the promoter region of the TNFα gene (−308 A/G) is associated with reduced weight loss in obese Australian subjects on an energy restricted diet. 189 healthy subjects and 91 subjects with type II diabetes were genotyped for the −308 Nco I polymorphism u...

Two carbonyl pseudohalogen compounds CF2HC(O)NCO and CF2HC(O)NCS have been synthesized and fully characterized by IR (gas-phase, matrix-isolation), Raman (liquid), UV–vis (gas-phase), and NMR (19F, 13C, 1H) spectroscopy. The conformational properties and photochemistry of both species have been...

A methodology for combining multi-parametric programming and NCO tracking is presented in the case of linear dynamic systems. The resulting parametric controllers consist of (potentially nonlinear) feedback laws for tracking optimality conditions by exploiting the underlying optimal control swit...