The proton affinities of methionine, methionine sulfoxide and their derivatives (methionine methyl ester, methionine sulfoxide methyl ester, methionine methyl amide, methionine sulfoxide methyl amide, N-acetyl methionine, N-acetyl methionine sulfoxide, N-acetyl methionine methyl ester, N-acetyl ...

Methionine is mostly produced synthetically. Thus, impurities are synthesis by-products in addition to oxidation and dimerization products. Here, a sensitive HPLC method for the determination of impurities in l-methionine was developed and validated using a SIELC® Primesep 100 column. Impurities...

ABSTRACTThe objective of current study was to investigate the effect of N-acetyl-l-methionine (NALM) supplementation on lactation performance and plasma variables in mid-lactating dairy cows. Forty-eight multiparous cows were blocked into 12 groups based on parity, days in milk, and milk product...

Here, a conventional chiral amino acid analysis method using high-performance liquid chromatography was coupled with a sample pretreatment using l-methionine γ-lyase from Pseudomonas putida ICR 3460 for the selective analysis of l-methionine and l-tryptophan. The sample was analyzed after the d...

Neutral amino acids can be delivered into cells through the l-type amino acid transporter-1 (LAT1), which is a sodium independent transporter. The LAT1 protein is expressed in different tissues, including kidney, blood brain barrier and intestinal wall hence LAT1 can be used as a target in disea...

To prepare labeled precursors for biosynthetic studies, methods for the specific introduction of tritium and deuterium into the reducing and the terminal glucose unit of maltotriose were developed. Thus [6″-3H]- and (6″-2H)-maltotriose (17) and (18) were prepared via selective methoxytritylati...

A maltotriose-producing α-amylase from Thermobifida fusca NTU22 was purified 7.2-fold as measured by specific activity from crude culture filtrate by ammonium sulfate fractionation, Sepharose CL-6B and DEAE-Sepharose CL-6B column chromatography. The overall yield of the purified enzyme was 22%....

We have used measurements of the phosphorescence intensity decay of the triplet probe erythrosin B, dispersed in amorphous glucose, maltose, and maltotriose at probe:sugar mole ratios of ∼1:104, to monitor the molecular mobility of the sugar matrix in the glass and melt around the glass-transit...

Acarbose, a pseudomaltotetraose, is produced by strains of the genus Actinoplanes. The compound is an inhibitor of α-glucosidases and is used in the treatment of patients suffering from type II diabetes. The benefits of acarbose for the producer are not known; however, a role as carbophor has b...

Pullulan production was carried out at shake-flask level and purified with isopropanol precipitation using single-step purification strategy. Purified pullulan obtained was used for preparing maltotriose syrup using pullulanase. Pullulanase was subjected to kinetic and thermodynamic characteriza...

Recombinant proteins are commonly expressed in prokaryotic expression systems for large-scale production. The use of genetically engineered affinity and solubility enhancing fusion proteins has increased greatly in recent years, and there now exists a considerable repertoire of these that can be...

In this study, we prepared maltotriose from the fermentation broth of Auerobasidium Pullulans CJ001 isolated from the sea mud by hydrolysis of pullulan with pullulanase. The fermentation broth was centrifuged to remove the microorganisms and then hydrolysed by pullulanase. The optimal hydrolysis...

The infrared spectra of carbamic acid: aryl-, alkyl esters in CCl4 and CS2 solution have been studied. It has been found that those N-phenyl carbamates containing an ortho halogen atom (F, Cl and Br) or φ group form intramolecular hydrogen bonds between the N-H proton and the unshared pair of e...

Equilibrium surface pressure-area isotherms of sodium docosyl sulfate (NaDS) monolayers have been measured on substrates containing various concentrations of sodium dodecyl sulfonate (SDSo). From these isotherms, saturation adsorptions of SDSo for various surface concentrations have been calcula...

Surface pressure-area isotherms have been determined for the octadecanol-docosyl sulfate monolayer system on 0.1 M potassium chloride solution. Four different temperatures in the range 288 to 318°K and 11 different monolayer compositions were investigated. The Goodrich method was used to calcul...

The evaporation resistances of monolayers of the octadecanol-docosyl sulphate system have been measured by the Langmuir-Schaefer technique. Eleven different compositions spanning the entire composition range were studied. At surface pressures above 15 mN m−1 evaporation resistances of the mixed ...

Surface pressure-molecular area isotherms of mixed Langmuir monolayer films of N-docosyl-N′-methyl viologen (C22MV2+) with arachidic acid were measured at the air/water interface and the electrochemical behavior of the films were investigated after transfer to an indium tin oxide electrode in a...

The real (J′) and imaginary (J″) components of the complex compliance have been measured between 24 and 100 cycles/sec. at 59.0°C. and 65.2°C. for poly-n-docosyl methacrylate. The results, reduced to 62°C., provide the relaxation and retardation spectra over a short region which encompasses...

The adsorption of radioactive calcium ions (45Ca2+) has been investigated in competition with cesium, sodium, or lithium cations. The model system consisted of the monolayers of sodium docosyl sulfate spread on buffered aqueous supports. The monolayers were of different surface concentrations of...

Transferred Langmuir-Blodgett layers of N-docosyl pyridinium, TCNQ have been exposed to different gaseous streams. Their reactions have been observed through a direct measurement of their electrical properties and through u.v.-visible spectroscopy.Bromine, iodine, nitrous oxide and ammonia affec...