LFchimera is a chimerical peptide containing Lactoferricin and Lactoferrampin antimicrobial peptides of bovine lactoferrin, and it has stronger bactericidal activity. Antimicrobial peptides (AMPs) like LFchimera have great potential as an alternative candidate for conventional antibiotics. Plant...

Plant cells have a variety of defense mechanisms to alleviate the deleterious effects of oxidative stress. The present work elucidated a schematic diagram of the proposed pathway of peanut hairy root tissue treated with different elicitors; paraquat (PQ), methyl jasmonate (MeJA), and cyclodextri...

ABSTRACTDiarrhea caused by Shigella, Salmonella, and Yersinia is an important public health problem, but development of safe and effective vaccines against such diseases is challenging. A new antigen delivery platform called bacterium-like particles (BLPs) was explored as a means for delivering ...

S-layers are paracrystalline planar assemblies of protein (or glycoprotein) which coat the surfaces of some gram-negative and gram-positive bacteria (eubacteria) and archaea (archaeobacteria). Over the past three to four years, high resolution three-dimensional structures of several S-layers hav...

FTIR spectroscopy was used for the characterization of S-layer proteins extracted from microorganisms isolated from kefir grains. S-layer from Lactobacillus brevis ATCC 8287 has been already characterized [G. Vidgren, I. Palva, R. Pakkanen, K. Lounatmaa, A. Palva, J. Bacteriol. 174 (1992) 7419] ...

The structure of the regular surface layer of Acetogenium kivui has been investigated by electron microscopy in conjunction with image processing techniques. From the averaged unit cell structure a tentative model of the subunit organization is derived. The occurrence of mono- and polycrystallin...

Shigella flexneri is a facultative intracellular bacterial pathogen that invades human colonic epithelial cells by a process called pathogen-induced phagocytosis. Pathogen entry requires three virulence plasmid-encoded proteins called invasion plasmid antigens (Ipa) B, C and D which are secreted...

A sequential injection (SI) system in the capillary format is reported for protein concentration determinations employing sample volumes in the microliter to nanoliter range. By incorporating a programmable syringe pump and computer control over the selection valve and data acquisition system, t...

Pull-down assays can identify members of protein complexes but suffer from co-isolation of contaminants. The problem is particularly acute when the specifically interacting partners are of low-abundance and/or bind transiently with low affinity. To differentiate true interacting partners from co...

Shigella rely on a type III secretion system as the primary virulence factor for invasion and colonization of human hosts. Although there are an estimated 90 million Shigella infections, annually responsible for more than 100,000 deaths worldwide, challenges isolating and stabilizing many type I...

Shigella spp. are food- and water-borne pathogens that cause shigellosis, a severe diarrheal and dysenteric disease that is associated with a high morbidity and mortality in resource-poor countries. No licensed vaccine is available to prevent shigellosis. We have recently demonstrated that Shige...

Förster resonance energy transfer (FRET) between the fluorescent ATP analogue 2′/3′-(N-methyl-anthraniloyl)-adenosine-5′-triphosphate (MANT–ATP) and enzymes is widely used to determine affinities for ATP–protein binding. However, in analysis of FRET fluorescence data, several important para...

A series of new 2-substituted [4-(1,3,4-oxadiazol-2-yl)methyl]phthalazin-1(2H)-one derivatives 7a–h to 9a–h were designed and synthesized from methyl (4-oxo-3,4-dihydrophthalazin-1-yl)acetate (4), which in was turn prepared from phthalic anhydride. The structure of synthesized new compounds we...

A new series of 4-amino-1-phenyl-5-pyrazolone based azo acid dyes (3a–g) has been synthesized from p- Sulphophenyl methyl pyrazolone (1). The synthetic methodology involved the nitrosation of p-sulphophenylmethylpyrazolone followed by reduction to 1-(p-sulphophenyl)-3-methyl-4-amino-5-pyrazolon...

The synthesis and characterization of some monomeric transition-metal coordination compounds with the novel bidentate ligand 5-methyl-4-(ethyl- mercaptomethyl)imidazole (abbreviated memi) are described, viz. [M(memi)2(H2O)2](NO3)2 and M(memi)2(NCS)2 with M = Co, Ni, Cu. Single crystals of [Ni(me...

A high-performance liquid chromatographic assay for the determination of N-[2(S)-(mercaptomethyl)-3-(2-methylphenyl)-1-oxopropyl]-l-methionine (SCH 42354; II), the active metabolite of the atriopeptidase inhibitor prodrug, N-[2(S)-(acetylthiomethyl)-3-(2-methylphenyl)-1-oxopropyl]-l-methionine e...

2,6-Bis(mercaptomethyl)pyridine (H2L) and its binuclear nickel(II), palladium(II) and platinum(II) complexes, containing the dianion as a bridging ligand, were prepared and characterized by physicochemical and spectroscopic data. Square-planar geometries are proposed for these divalent metal com...

The reactivity of a binuclear nickel(II) sulfur bridged complex with benzyl bromide and methyl iodide has been studied. The preparation of a novel binuclear nickel(II) bromine bridged complex of 2,6-bis[(benzyl)mercaptomethyl)]pyridine has been carried out from the reaction involving bis[2,6-bis...

A highly convenient and efficient one step procedure for the synthesis of polystyrene thiol resin from Merrifield resin is described. Potassium O,O-diethyl thionophosphate is used as a thiolating reagent for the conversion of halogen (chloro) group to thiol functionality in refluxing DMF. By a s...

The thiaminalisation of 2-amino-2-(mercaptomethyl)propane-1,3-diol [‘2-(hydroxymethyl)cysteinol’] with aryl(di)aldehydes is reported. The resulting new class of 2-aryl-4,4-bis(hydroxymethyl)-1,3-thiazolidines is investigated by NMR and IR spectroscopy in tandem with DFT calculations, permittin...