A commercially available high-performance gel solution, ‘Hydrolink Long Ranger’, was used for direct automated sequencing of PCR-amplified double-stranded 16S rDNA. The 1.5 kb rDNA fragments were amplified directly from bacterial cell lysates and sequenced by using Tth DNA polymerase with the ...

Human keratinocyte growth factor (KGF) is a recently identified mitogen for epithelial cells produced by normal stromal fibroblasts. KGF has been shown to stimulate keratinocyte migration and promote re-epithelialization of skin suggesting a critical role for KGF in wound healing. To understand ...

Human recombinant basic fibroblast growth factor (bFGF) is a potent mitogen for normal human dermal fibroblasts in the presence of heparin which binds to and stabilizes it. The optimal mitotic response is obtained with a concentration of 1 ng/ml of bFGF in monolayer cultures (non-differentiated ...

In most moth species, including Mamestra brassicae, pheromone biosynthesis activating neuropeptide (PBAN) regulates pheromone production. Generally, PBAN acts directly on the pheromone gland (PG) cells via its specific G protein-coupled receptor (i.e. PBANR) with Ca2+ as a second messenger. In t...

Insect pheromone biosynthesis activating neuropeptide (PBAN) controls the synthesis and actuating of sex pheromones of female adult. In the current examination, the full-length cDNA encoding the PBAN receptor was cloned from the pheromone gland (PG) of Antheraea pernyi (AntpePBANR). The AntpePBA...

Previous reports have shown that the LEW/N and F344/N inbred rat strains display a differential sensitivity to cocaine in a number of preparations, with the LEW/N rats displaying an increased sensitivity to both the reinforcing and aversive effects of cocaine (relative to the F344/N rats). Given...

We recently identified a single-nucleotide polymorphism in the Ncf1 gene, a component of the NADPH oxidase complex, to be the cause of one of the strongest identified loci for arthritis severity in rats. This polymorphism was found to be naturally occurring in a collection of inbred rat strains ...

Towards the eventual purpose of facilitating analyses of specificities and functions of LEW rat T lymphocytes involved in the induction and development of organ-specific autoimmune disorders, hybridoma cells expressing class I and class II MHC antigens of LEW rat have been developed. B cell hybr...

Although Fischer (F344) and Lewis (LEW) rats differ in their sensitivity to the rewarding effects of ∆9-tetrahydrocannabinol (THC), no data have been reported on differences in their sensitivity to the drug's aversive effects, a limiting factor in drug use and abuse. Examining the degree of...

The inbred Fischer (F344) and Lewis (LEW) rats, while originally developed as animal models for cancer and tissue transplantation research, have since been used to study genetic differences in a variety of physiological and behavioral endpoints. In this context, LEW rats show greater sensitivity...

We have investigated the effect on phospholipidic bilayers of LEW-10, a synthetic flavonoid, derivative of diosmin. Two optical techniques, Quasi-elastic Light Scattering (QLS) and Fourier Transform Infrared Spectroscopy (FT-IR) were used. The results show that in the presence of LEW-10, the pha...

Presently, modern pharmaceuticals, are almost exclusively derived from the arduous refining of petroleum whose supply is inherently unsustainable. In order to address this issue bio-based materials are increasingly being used for chemical synthesis, particularly in drug delivery systems. Biodegr...

Interactions between ionic liquid surfactant [C12mim]Br and DNA in dilute brine were investigated in terms of various experimental methods and molecular dynamics (MD) simulation. It was shown that the aggregation of [C12mim]Br on DNA chains is motivated not only by electrostatic attractions betw...

Watercress (Nasturtium officinale R. Br.), an aquatic leafy vegetable of the Brassicaceae family, is known as a nutritional powerhouse. Here, we de novo sequenced and assembled the complete chloroplast (cp) genome of watercress based on combined PacBio and Illumina data. The cp genome is 155,106...

SummaryEnvironment isotopes (δ18O and δ2H) and Cl/Br ratios in groundwater have been used to trace groundwater recharge and geochemical processes for arsenic contamination in Datong Basin. The arsenic concentrations of groundwater samples ranged from 0.4 to 434.9 μg/L with the average of 51.2...

The X-ray powder analysis, thermogravimetric analysis, differential scanning calorimetry analysis and complex impedance spectroscopic data have been carried out on (C8H12N)3SnBr6.Br compound. The results show that this compound exhibits a phase transition at (T=365±2 K) which has been character...

We present halogen compositions in a series of alkaline rocks from the Kaiserstuhl, Hegau and Urach areas of the Upper Rhine Graben region (South Germany). Most primitive rocks (olivine melilitites and olivine nephelinites) have lower Cl and Br concentrations (generally below 100 μg/g and below...

Novel osmium complexes (ThioH)2[OsCl6] (1) and (ThioH)2[OsBr6] (2) have been synthesized via the interaction of H2[OsX6] (X = Cl, Br) with thiourea (Thio) in concentrated HX and characterized by chemical analysis, potentiometry, IR- and UV–Vis spectroscopy, X-ray powder and single-crystal diffr...

In this work, the potassium 5-Br-2-isonicotinoyltrifluoroborate salt (B-ITFB) have been experimentally characterized by using FT-IR, FT-Raman and Ultravioleta-visible spectra while Ab-initio calculations in gas and in aqueous solution phases were employed to predict their structural and vibratio...

Six novel coordination polymers, namely, [Cu1.5(H2L)0.5(H4L)0.5(bibp)1.5]·3H2O (1), [Zn1.5L0.5(bib)(H2O)]·4H2O (2), [Zn1.5L0.5(bib)1.5]·DMF (3), [Co0.5(H4L)0.5(bibpip)(H2O)]·3H2O (4), [Zn(H2L)0.5(tip)] (5), [Cu(H4L)0.5(H6L)0.5(tip)(H2O)]·2H2O (6), [H6L = p-terphenyl-2,2″,2′′′,5,4″,4′′...