To evaluate the biosafety of the plasmid pcDNA3-1E of Eimeria acervulina in chicken, two-week-old chickens were injected intramuscularly with the plasmid pcDNA3-1E at dose of 50 μg/chicken. At the 15 days post-injection, the tissue samples were collected, the total DNA was extracted, and the 3-...

Steviol glycosides from Stevia rebaudiana leaves are used in stevia-based sweeteners for their intense sweetness and low calories. Rebaudioside D is present in leaves in minute quantities (∼0.4–0.5% w/w total dry weight), but it is ∼350 times sweeter than sucrose, and sweeter than the more ab...

A technique for the concurrent on-site quantitative analysis of gold (Au), palladium (Pd), or platinum (Pt) in the aqueous acidic matrix has been proposed. The Au, Pd, or Pt in the matrix was selectively concentrated in macrocycle-equipped solid-phase extraction (SPE) system, followed by analysi...

End-of-life electrical and electronic equipment is the potential secondary resource for economically-viable precious metals (PMs), e.g., gold (Au), palladium (Pd), or platinum (Pt). The hydrometallurgical processes produce acidic leachates during the recovery of PMs from waste sources, while the...

A leaching process for platinum contained in a magnetite ore was studied, as an alternative process to cyanidation and aqua regia digestion. The proposed process employs concentrated chloride solutions and ozone gas injection during the entire leaching process. The effect of pH, chloride concent...

4-Alkyl-substituted phenols and aromatic ethers were comparatively fluorinated with electrophilic fluorinating reagents such as cesium fluoroxysulfate (CFS), Selectfluor™ F-TEDA-BF4, and Accufluor™ NFTh in MeCN or MeOH. Reactions resulted in the formation of three types of products: 2-fluoro-4-a...

A series of 1H-1,2,3-triazolyl piperazino oxazolidinone analogs with optionally varied glycinyl substitutions were synthesized and their antibacterial activity assessed against a panel of susceptible and resistant Gram-positive and selected Gram-negative bacteria including clinical isolates. The...

This paper describes an innovative fast and multipurpose method for the chemical inspection of meat and fish products by liquid chromatography-tandem mass spectrometry. Solid-liquid extraction and low temperature partitioning were applied to 17 analytes, which included large bacteriocins (3.5 kD...

Treatment of 3-O-acetyl-5-deoxy-5-(dimethoxyphosphinyl)-1,2-O-isopropylidene- α-d-glucofuranose (7) with dihydropyran in the presence of pyridinium p-toluenesulfonate gave the 6-O-(tetrahydropyran-2-yl) derivative in 91% yield. Ring-enlargement of this compound by the known, 2-step procedure ga...

The synthesis of a series of hydroxyphosphinyl-containing analogues of the HMG-CoA reductase inhibitor trans-tetrahydrocompactin is described.

Arginine vasopressin (AVP) activates three vasopressin receptors and it also has an agonistic activity on the oxytocin receptor. For an accurate description of the target receptor subtype(s) responsible for complex AVP and oxytocin actions, a careful evaluation of ligand specificity and receptor...

A novel stilbene-ether type of photolabile protecting group (PPG) for hydroxyl group has been developed. It contains a stable aryl ether group in the protected form and can be deprotected by acid-catalyzed photorearrangement under 300-nm irradiation. The selective deprotection has also been achi...

The local segregation and dynamics of poly(propyl methacrylate) (PPMA) are studied as a function of temperature by X-rays and by dielectric spectroscopy and rheology, respectively. Pressure is employed in dielectric spectroscopy for investigating the origin of segmental dynamics and for separati...

TGA/FTIR was employed to investigate the thermal degradation mechanism of two types of self-curable Polyurethanes obtained from isophorone diisocyanate (IPDI), Poly(propyleneglycol) (PPG), and (trimethoxysilyl) propyl isocyanate and N-butyl 3-trimethoxysilyl propyl amine respectively. The TGA/FT...

ObjectiveHere we develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of digoxin in serum.

The low therapeutic index of digoxin necessitates careful monitoring of its serum levels. Most of digoxin immunoassays suffer from interferences with digoxin-like immunoreactive substances. Since aptamers have been shown to be highly specific for their targets, the aim of this study was to devel...

Rhodamine dyes are widely used as molecular probes in different fields of science. The aim of this paper was to ascertain to what extent the structural peculiarities of the compounds influence their absorption, emission, and acid-base properties under unified conditions. The acid-base dissociati...

Ascidiacyclamide [ASC, cyclo(-Ile-oxazoline-d-Val-thiazole-)2] is a cyclic octapeptide isolated from tunicates. We designed ASC analogues [cyclo(-Ile-Xxx-d-Val-thiazole-)2] in which Pro or a homologue was substituted for oxazoline: [Pro]ASC (Xxx: proline), [Aze]ASC (Xxx: (S)-Azetidine-2-carboxyl...

A selective and sensitive high performance liquid chromatography–electrospray ionisation–mass spectrometry method has been developed for the determination of balofloxacin (BLFX) in human plasma. The sample preparation was a liquid–liquid extraction, and chromatographic separation was achieved...

A novel method of luminescence enhancement effect for the determination of balofloxacin (BLFX) was proposed. A new system of the BLFX–Eu3+–SDBS (sodium dodecylbenzene sulfonate) was investigated. It was found that SDBS significantly enhanced the luminescence intensity of the BLFX–Eu3+ complex...