A method for cytophotometric quantifications of retrograde axonal transport of a fluorescent tracer in tissue sections is described. As a fluorescent tracer the anionic vital stain primuline proved to be suitable since it resulted in a strong yellow-green fluorescence, which faded very slowly pe...

The origin of the centrifugal fibers to the olfactory bulb in the goldfish was examined by injecting a fluorescent dye, primuline, into the olfactory bulb by means of hydraulic pressure. Three to five days after the primuline injection, which was confined to the unilateral olfactory bulb, a larg...

The hepatitis C virus (HCV) multifunctional nonstructural protein 3 (NS3) is a protease that cleaves viral and host proteins and a helicase that separates DNA and RNA structures in reactions fueled by ATP hydrolysis. Li et al. (2012) recently synthesized a series of new NS3 helicase inhibitors f...

Phosphatidylethanolamines (PEs) and phosphatidylglycerols (PGs) are abundant lipid constituents of the membranes of Escherichia coli. The reaction between these lipids and hypochlorous acid (HOCl), an important constituent of disinfectants, was investigated by combined thin-layer chromatography ...

The fabrication of fluorescence indicator/layered double hydroxide (LDH) ultrathin films (UTFs) by alternate assembly of Primuline and Mg-Al LDH nanosheets using the layer-by-layer (LBL) deposition technique has been reported, and their application as a raitometric fluorescence chemosensor for H...

In prion diseases, infectious pathogenic particles that are composed of abnormal prion proteins (PrPSc) accumulate in the brain. PrPSc is biochemically characterized by its protease-resistance core (PrPres), but its structural features have not been fully elucidated. Here, we report that primuli...

The preparation of [Zn{SCH2CH2N(CH3)2}2] by electrochemical oxidation of zinc and simultaneous reduction of 2-(dimethylamino)ethanethiol disulphide in an acetonitrile solution containing triethylamine is described. The compound crystallizes in the orthorhombic space group P212121 with a = 22.010...

A series of 9-(alkylthiomethyl)-10-hydroxycamptothecins and pyrano-fused camptothecin derivatives were synthesized via the reaction of topotecan hydrochloride with various thiols and alkyl vinyl ethers respectively. In the reactions, topotecan hydrochloride was utilized as ortho-quinonemethide (...

N-nonanoyl vanillylamide (1) is a synthetic substitute of capsaicin (2), a pungent principle in red pepper. 1 was reacted with succinic anhydride, 3-chloro-1,2-propanediol, sodium chloroacetate, and 2-chloroethanol to furnish N-nonanoyl vanillylamide-4-succinyl ester (3), N-nonanoyl vanillylamid...

A new, one-pot convenient method for the synthesis of a variety of β-phosphonomalonates by a tandem Knoevenagel–phospha-Michael reaction of phosphite esters with aryl/heteroaryl/alkyl aldehydes and malonitrile in an aqueous micellar solution of sodium stearate is described. This method offers ...

Measurements of propyl stearate monolayers were made using a new laser light scattering apparatus. Thermal capillary waves in the frequency range 7–16 kHz were studied. For the first time the film elasticity and viscosity have been determined from light scattering measurements. The determined e...

An extracellular lipase partially purified from Bacillus thermoamylovorans BHK67 was effectively immobilized onto modified magnetic MgFe2O4 nanoparticles (NPs). NPs were prepared by the sol-gel auto-combustion method and characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diff...

The present work is devoted to the magnetic behaviour of Mn12-stearate single-molecule magnets ([Mn12O12(CH3(CH2)16CO2)11(CH3CO2)5(H2O)4]·2CH3COOH) immobilized inside the SBA-15 mesoporous silica. The stearate’s complexed Mn12, in contrast to the commonly used Mn12-acetate, is soluble in most ...

The present work deals with the preparation of some oil sorbers based on cellulose derivatives to control petroleum oil spills. In this respect, hydroxypropyl cellulose HPC was used to synthesize hydroxypropyl cellulose acrylate HPCA macromonomer by esterification of HPC with acryloyl chloride. ...

Different concentrations of graphene oxide (GO) were successfully embedded into poly (vinyl chloride-co-vinyl acetate-co-2-hydroxypropyl acrylate) (PVVH) copolymer. The composites obtained were characterized using XRD, FT-IR, SEM and UV/Vis techniques, in addition to investigating their electric...

High enantioselectivity (E 94) was observed in Candida antarctica lipase B-catalyzed hydrolysis of the corresponding acetate of racemic title compound. The reaction rate of the slow (S)-isomer was effectively suppressed by lowering the reaction temperature from 25 °C to 5 °C, to allow a five t...

Trimethylsilylcyanide was added to various imines derived from (2S)-ethyl lactate in the presence of Lewis acids to provide both syn- and anti- β-hydroxy-α-aminonitrile stereoisomers. Syn-products were found to be the major in most instances, however, anti-products were formed in good yield an...

Novel approach to CF3-/CHF2-oxazoles was developed. The key reaction was oxidation of fluoroalkyl enamides with PhI(RCO2)2.

The synthetic sequence to make 4-[4′-bis(2″-chloroethyl)aminophenyl]-4,4-difluorobutanoic acid {4,4-difluorochlorambucil} was not taken beyond the intermediate stage involving methyl 4,4-difluoro-4-(4′-aminophenyl) butanoate, since this amine was hydrolytically unstable. Reaction of methyl 4-...

During the search for new antivirals, various N,N′-bis-5-pyrimidyl derivatives of 3,12-diaza-6,9-diazonia(5,2,5,2)dispirohexadecane dichloride (dispirotripiperazine) were synthesized. To reveal relationships between chemical structure and antiviral activity, the compounds were characterized by ...