Shark cartilage chondroitin surfate C was fractionated by chromatography on Sepharose CL-4B-2.5 to 1.5m ammonium sulfate in 10mm hydrochloric acid at 4°. Both unit-disaccharide composition and molecular-size distribution clearly affected the fractionation. Comparison of this fractionation with ...

Quantification of the octyl content in Octyl-Sepharose CL-4B was accomplished by three independent methods. Firstly, the 1H NMR spectrum was registered on a deuterium chloride hydrolysed gel. Secondly, gas chromatography was applied to the ether-linked ligands cleaved by boron tribromide. Finall...

The retenion of homologous aliphatic alchohols and carboxylic acids in dilute phosphate buffer solution was measured on octyl-Sepharose CL-4B and on unsubstituted Sepharose CL-4B as a function f chaing lenghts, pH and temperature. From the retention data the standard thermodynamic function (Δ°...

The release of ligands from Phenyl-Sepharose™ CL-4B, Phenyl-Sepharose™ FF and Phenyl-Superose™ has been studied in bulk and column experiments. The leakage products have been identified and quantified by liquid chromatography, fluorescence spectroscopy and proton NMR spectroscopy. It is demonstr...

This paper evaluates the immunopurification behavior of a plantibody HBsAg specific plantibody coupled to Sepharose CL-4B at different ligand densities. Results show no significant differences in the adsorption and elution capacities, and rHBsAg recovery of immunosorbents at 3.43, 4.45, and 5.31...

Heparin was fractionated by chromatography on Sepharose CL-4B/3.8–2.0 M ammonium sulphate in 0.01 M hydrochloric acid at 4°C based on a principle different from that of gel filtration —possibly due to multiple interaction mechanisms including those based on hydrophobic bonds. The results of t...

(2R,3S)-4-Halo-3-benzyloxy-2-(N-methoxycarbonyl-N-benzylamino)butyronitriles have been prepared through an efficient three-step sequence from (2R,3S)-2-benzylamino-3-benzyloxy-4-(tert-butyldimethylsilyloxy)butyronitrile, which is readily available in diastereomerically pure form by a Strecker-ty...

A widespread exploration on the intra-molecular charge transfer interaction through an efficient π-conjugated path from a strong electron-donor group (amino) to a strong electron-acceptor group (nitro) has been carried out using FTIR, FT-Raman, UV–Vis, fluorescence and NMR spectra on insectici...

A DMAP catalyzed synthesis of new 4-benzyloxy- and 4-aryloxy-2-trichloromethylquinazolines was studied, in a view to react 4-chloroquinazolines with poorly nucleophilic alcohols such as benzylic alcohols, via a simple and cheap SNAr reaction approach. A fast (1 h) general operating procedure, af...

Liquid–liquid equilibrium (LLE) data of the solubility (binodal) curves and tie-line end compositions were examined for mixtures of {(water (1) + butyric acid (2) + diethyl succinate or diethyl glutarate or diethyl adipate (3)} at 298.2 K and 101.3 ± 0.7 kPa. The relative mutual solubility of ...

The two cyclic acetals, 2,3-di-O-methylene-l-threitol and dimethyl 2,3-di-O-methylene-l-threarate, were used for the synthesis of two series of PBS copolyesters differing in which unit, butylene or succinate, was replaced, in addition of the corresponding parent homopolyesters. Polycondensation ...

Succinic acid derived from sugar is reportedly going to be an abundant and inexpensive feedstock for polymer synthesis in the near future. This article reports on succinic acid based polyester polyols prepared with butane diol and compares them to polybutylene adipate, a common polyester polyol ...

The purpose of this study was to use statistical design of experiments to develop a stable aqueous enteric coating formulation containing stabilizing excipients, such as polyethylene glycol that can minimize hydroxypropyl methylcellulose acetate succinate aggregation and minimize spray-nozzle cl...

Phthalate diesters such as di (2-ethylhexyl) phthalate (DEHP) are considered ubiquitous contaminants and are poorly biodegraded in the environment. Moreover, both the parent compound and stable metabolites such as mono (2-ethylhexyl) phthalate (MEHP) are linked to several negative impacts on the...

Hydroxypropyl methylcellulose acetate succinate (HPMCAS) has been widely used in amorphous solid dispersions and as an enteric coating polymer. Under aqueous coating conditions and at elevated coating temperatures, HPMCAS particles tend to aggregate and clog the spray-nozzle, hence interrupting ...

Different (co)polyesters, poly(1,4-butylene succinate-ran-2,3-butylene succinate) (PBB’S), were enzymatically copolymerized at different 1,4-butanediol (1,4-BDO)/2,3-butanediol (2,3-BDO) molar ratios, with immobilized Candida antarctica lipase B (CALB). The synthesized macromolecular architectu...

Diethyl succinate is continuously produced from succinic acid and ethanol in a 6-m tall, elevated pressure reactive distillation column. Esterification is carried out in excess ethanol such that water and ethanol are produced in the distillate and diethyl succinate is produced in the bottoms str...

The transesterification between poly(ethylene terephthalate) (PET) and dibutyl succinate functionalized polyethylene (POF) was studied by preparing blends in a discontinuous mixer in the presence of different Zn and Ti catalysts. In particular the catalytic activities of Zn(OOCCH3)2, Ti(OBu)4, Z...

The isobaric vapor–liquid equilibrium (VLE) data for three binary systems of dimethyl succinate (DMS) + dimethyl glutarate (DMG), dimethyl succinate + dimethyl adipate (DMA) and dimethyl glutarate + dimethyl adipate were determined at 2, 5.2 and 8.3 kPa with a dynamic recirculating apparatus an...

4-Anilino-5-phenyl-4H-1,2,4-triazole-3-thiol (1) reacted with formaldehyde and different amines to give Mannich bases 2a–i. Treatment of compound 1 with formaldehyde afforded the corresponding 2-hydroxymethyl derivative 3, which upon its reaction with thionyl chloride yielded the corresponding ...