Although less common than abuse of marijuana and cocaine, abuse of magic mushroom, peyote cactus, lysergic acid diethylamide (LSD), khat, and volatiles is observed in the United States. Eating wild mushroom is dangerous because misidentification of toxic mushroom as magic mushroom may be fatal. ...

A liquid chromatographic procedure with electrospray ionization tandem mass spectrometric detection has been developed and validated for LSD and iso-LSD determination. A one-step liquid–liquid extraction on 1 ml blood or urine was used. The lower limit for quantitative determination was 0.02 μ...

A liquid chromatography mass spectrometric (LC/MS/MS) method has been developed for the determination of LSD, iso-LSD and the metabolite 2-oxo-3-hydroxy-LSD in forensic applications. The procedure involves liquid–liquid extraction of the analytes and LSD-D3 (internal standard) from 1.0 g whole ...

BackgroundNumerous anecdotal reports suggest that repeated use of very low doses of lysergic acid diethylamide (LSD), known as microdosing, improves mood and cognitive function. These effects are consistent both with the known actions of LSD on serotonin receptors and with limited evidence that ...

Three case reports are presented, including autopsy findings and toxicological screening results, which were tested positive for the potent hallucinogenic drug lysergic acid diethylamide (LSD). LSD and its main metabolites were quantified in brain tissue and femoral blood, and furthermore hemato...

Seventy-four urine specimens previously found to contain lysergic acid diethylamide (LSD) by gas chromatography–mass spectrometry (GC–MS) were analyzed by a new procedure for the LSD metabolite 2-oxo-3-hydroxy-LSD (O-H-LSD) using a Finnigan LC–MS–MS system. This procedure proved to be less c...

Lysergic acid diethylamide (LSD) is administered in low dosages, which makes its detection in biological matrices a major challenge in forensic toxicology. In this study, two sensitive and reliable methods based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) were established and ...

The reaction of dimethyl 2-(tosylmethyl)fumarate (6), prepared by iodosulfonylation-dehydroiodination of dimethyl itaconate, with carbonucleophiles or sodium methoxide allows the direct synthesis of 3-substituted itaconate ester derivatives 8 via a SN2′ pathway. When sodium thiolates are used a...

Isomeric mixed methyl ethyl esters of 2-methylmaleic acid (citraconic acid) show different electron ionization (EI) mass spectra. Methyl radical is exclusively lost from the M+ ion of one isomer ((Z)-methyl 3-ethoxycarbonyl-2-butenoate), while the elimination of methanol is more efficient in the...

The addition of Et2AlCN in the presence of ZnBr2 or Et2AlCl to 1,3-dicarbonyl compounds derived from (S)-4-isopropyl-2-oxazolidinone, proceeds with high diastereoselectivity (94–98% de) and good chemical yields. This type of addition to chiral β-dicarbonyl substrates represents a new synthetic...

A reaction of Grignard reagents with an optically pure N-sulfinylimine derived from methyl 2-formylbenzoate yields enantioenriched isoindolinones and tert-butyl sulfoxides. The products are formed by the addition of the nucleophile to N-sulfinylimine followed by cyclization to form N-tert-butyls...

N-ε-(indole-3-acetyl)-l-Lysine (ε-IAA-Lys) and its N-α-acetyl derivative (Ac-ε-IAA-Lys), products of the metabolism of indole-3-acetic acid (IAA) in Pseudomonas syringae subsp. savastanoi, have been synthesized by a conjugation of IAA with the N-α-CBz-l-lysine p-nitrophenylester. The same s...

N′-Propylhydrazide analogs of hydroxamic inhibitors of histone deacetylases (HDACs), including tubastatin A, vorinostat and belinostat, were synthesized. All prepared compounds inhibited HDAC1/2/3, but not HDAC6, except for one hydrazide analog of HDAC4/5/7 inhibitor that was completely inactiv...

SummaryRecent studies of N-terminal acetylation have identified new N-terminal acetyltransferases (NATs) and expanded the known functions of these enzymes beyond their roles as ribosome-associated co-translational modifiers. For instance, the identification of Golgi- and chloroplast-associated N...

A product of indole-3-acetic acid (IAA) metabolism having an auxin-like activity has been isolated from liquid cultures of Pseudomonas syringae pv. savastanoi. By spectral data and chemical correlations the compound has been identified as α-N-acetyl-indole-3-acetyl-ε-l-lysine (Ac-IAA-Lys). The...

A kinetic model of the process response of nervous tissue to an external signal stimulus is proposed. The model is based on the multistage and non-linear nature of the dynamic process of changes of N-acetylaspartate concentration. The existence of multiple steady states explains the trigger effe...

Protein amino (N) termini are major determinants of protein stability in the cytosol of eukaryotes and prokaryotes, conceptualized in the N-end rule pathway, lately referred to as N-degron pathways. Here we argue for the existence of N-degron pathways in plastids of apicomplexa, algae, and plant...

Aminoacylases (EC 3.5.1.1.4) of Streptomyces ambofaciens ATCC 23877 were immobilized by chemical and physical adsorption onto SBA-15 mesoporous silica materials. The activity of the immobilized aminoacylases was firstly evaluated by considering the hydrolysis reactions of N-α-acetyl-lysine, use...

A series of novel benzisothiazolylpiperazine derivatives combining potent dopamine D2 and D3, and serotonin 5-HT1A and 5-HT2A receptor properties were synthesized and evaluated for their potential antipsychotic properties. The most-promising derivative was 9j. The unique pharmacological features...

Ciprofloxacin (CP), an antibiotic has been shown to have antiproliferative and apoptotic activities in several cancer cell lines. Moreover, several reports have highlighted the interest of increasing the lipophilicity to improve the antitumor efficacy. These studies have led us to synthesize new...