The addition of glycerol, sucrose, or other diol-containing reagents to solutions of aminoacyl-tRNA (aa-tRNA) substantially increased the rate of hydrolysis of the aminoacyl ester bond. Glycerol at 4.9% (vv) doubled the rate of deacylation ofr several aa-tRNAs and peptidyl-tRNAs, including fMet-...

A method has been developed to measure the specific radioactivity of blood or plasma glycerol under conditions when glycerol label is only a very small fraction of total nonionic label. Glucose is removed from a deproteinized extract by glucose oxidase (EC 1.1.3.4.) and catalase (EC 1.11.1.6.), ...

A phosphatidyl-group from either phosphatidyl glycerol or diphosphatidyl glycerol is covalently bonded to diglucosyl diglyceride to form phosphatidyl diglucosyl diglyceride in a reaction readily catalyzed by a disrupted membrane enzyme preparation from Streptococcus,faecalis.

The permeability coefficients for urea, thiourea, glycerol and glucose were determined for full thickness nude mouse skin and the dermis of nude mice by means of an infinite dose diffusion cell system in vitro. The permeability coefficients were found to be very low and ranged between 1.0 × 10−...

The presented study describes the synthesis, pharmacological evaluation (AChE and BuChE inhibition, beta amyloid anti-aggregation effect and neuroprotective effect), molecular modeling and crystallographic studies of a novel series of isoindoline-1,3-dione derivatives. The target compounds were ...

Hepatocytes prepared from overnight-fasted rats were incubated for 120 minutes in the presence of 2.5 mmol/L [1,3-13C]glycerol-1,2,3-tris(methylsuccinate) or glycerol-1,2,3-tris(methyl[2,3-13C]succinate). The identification and quantification of 13C-enriched metabolites by a recently developed m...

The metabolism of 14C-labeled glyceryl trinitrate was studied in the rat. Absorption, tissue distribution and elimination were followed after administering a single dose by gavage. Thin-layer chromatography and radio-scanning methods were employed to assay urinary drug metabolites, including the...

Glycerol kinase activity and glycerol utilization by rat granular pneumocytes were determined in order to investigate the rate-limiting step for glycerol incorporation into lung lipids. Granular pneumocytes were isolated in primary culture following trypsinization of rat lungs. Glycerol kinase a...

In renal tubules isolated from fed rabbits glycerol is not utilized as a glucose precursor, probably due to the rate-limiting transfer of reducing equivalents from cytosol to mitochondria. Pyruvate and glutamate stimulated an incorporation of [14C]glycerol to glucose by 50- and 10-fold, respecti...

Intestinal mucosal microsomes from several animal species (non-fasted) were used to study the formation of glycerolipids from sn-[1,3-14C]glycerol 3-phosphate and palmitoyl-CoA. Rates of glycerolipid biosynthesis were species dependent, since mouse and rat were quite low compared to hamster, gui...

Publisher SummaryThis chapter provides the basic synthesis paths routes for the manufacture of prothiocarb, with a listing of the main raw materials and intermediates used in the synthesis of prothiocarb. Prothiocarb is commercially known as Previcur or Dynone, and it belongs to the thiocarbamat...

Complex formation between oxovanadium(IV) and several pyridinecarboxylic acids (picolinic acid, 3-hydroxypicolinic acid and 2-hydroxynicotinic acid) was studied in aqueous solution by pH-potentiometric and spectroscopic (electron paramagnetic resonance and electronic absorption) techniques. The ...

The reaction of dimethylthallium(III) hydroxide with picolinic acid (Hpic), 3-hydroxypicolinic acid (H23hpic) and 6-hydroxypicolinic acid (H26hpic) in an aqueous/methanol mixture afforded the complexes [TlMe2(pic)] (1), [TlMe2(H3hpic)] (2) and [TlMe2(H6hpic)] (3), respectively. Complex 3′, [NaT...

Cobalt(II) complexes of 6-hydroxypicolinic acid (6-OHpicH), namely [Co(6-OHpic)2(H2O)2] (1) and [Co(6-OHpic)2(4-pic)2]·4-pic (2), and of 2-hydroxynicotinic acid (2-OHnicH), [Co(2-OHnic)2(H2O)2] (3) were prepared. The crystal structures of free 6-hydroxypicolinic acid monohydrate 6-OHpicH·H2O (...

Three new lanthanide complexes, [La(HpicO)3(phen)(H2O)]n (1) and Ln3(picO)4(phen)3(H2O)2·HpicO·0.5H2O (Ln=Er (2), Yb (3)) were synthesized by the hydrothermal reactions and characterized by single crystal X-ray diffraction, elemental analysis and IR spectrum. In complex 1, each La(III) ion is ...

The reaction of trans-[ReOBr3(PPh3)2] with 6-hydroxypicolinic acid (H2hpa) in ethanol led to the isolation of the ReIVReIV dimer (μ-O)(μ-hpa)2[Re2Br(OEt)(PPh3)2] (1). Each hpa2− anion acts as a bridging ligand with the coordination of a neutral pyridol oxygen to one rhenium ion, and the coordi...

A concise, chiral auxiliary free, methodology for the preparation of orthogonally protected Cα-Methyl Cysteine has been developed. Curtius rearrangement of malonate halfester from PLE hydrolysis (J. Pept. Sci. 2008, 14, 1151; J. Org. Chem. 2003, 68, 5403) and simultaneous Fmoc protection using ...

The 4,4′,4′-tris(benzoyloxy)trityl (TBTr) group was introduced into the amino groups of the common deoxyribonucleosides by means of the transient protection using the trimethylsilyl group. The TBTr group introduced onto the N6 -amino group of deoxyadenosine was found to have considerable retar...

A series of novel 4-chlorophenyl N-alkyl phosphoramidates of 3′-azido-2′,3′-dideoxy-5-fluorouridine (12–21) were synthesized by means of phosphorylation of 3′-azido-2′,3′-dideoxy-5-fluorouridine (4) with 4-chlorophenyl phosphoroditriazolide (10) followed by a reaction with the appropriate...

Convenient synthesis of 2-amino-1,5-disubstituted and 2-amino-1,4,5-trisubstituted imidazoles has been reported using readily available starting materials and simple reagents under mild conditions. Guanylation of 1-amidino-3-trityl-thioureas 1 and 7 using mercury(II) chloride (Caution) as a thio...