Cytokines and reactive oxygen species are inflammatory mediators that lead to increased sensitivity to painful stimuli, and their inhibition represents a therapeutic approach in controlling acute and chronic pain. The water-soluble flavonone hesperidin methyl chalcone (HMC) is used in the treatm...

A subchronic toxicity study of methyl hesperidin was performed using B6C3F1 mice. The flavonoid was administered to groups often males and ten females in dietary levels of 0, 0.3, 0.6, 1.25, 2.5 and 5.0% for 13 weeks. No significant treatment-related differences were found in data for body weigh...

There are global increased interests to identify novel agents that can possess anti-tumor effects or maximize the anti-tumor effects of low doses for conventional anti-cancer drugs. The aim of this study was to investigate anti-tumor effects and protective role of isolated hesperidin from sweet ...

Nano-carriers enhance drugs stability and solubility and prevent their rapid degradation. Silver nanoparticles (AgNPs) are preferable for drug delivery applications due to their unique properties and less toxicity. Protein AgNPs are versatile nano-carriers as they provide the anchoring layer for...

The aim of our study was to evaluate the hesperidin anti-hyperglycemic and hypo-lipidemic effects on diabetic myocardial infraction (DMI) rats by decreasing the blood glucose and blood cholesterol contents. The object of the study was to examine the 7groups of male Wistar rats, each group contai...

The interaction of Methyl hesperidin (MH) with Buman serum albumin was studied by spectroscopic methods including Fluorescence quenching technology, UV absorbance spectra and Fourier transform infrared (FT-IR) spectroscopy under simulative physiological conditions. The result of fluorescence tit...

A novel amorphous solid dispersion (ASD) of poorly water-soluble nobiletin (Nob) with highly water-soluble methyl hesperidin (MeHes) was developed. Mixtures of Nob and excipients (MeHes, cellulose derivatives, and synthetic polymers) were processed by hot-melt extrusion (HME). Powder X-ray diffr...

Publisher SummaryThis chapter discusses the pathways of biosynthesis of the guanidinated inositol moieties of streptomycin and bluensomycin. Studies in vivo on the biosynthesis of streptomycin by intact mycelia have provided important guideposts for enzymic studies with cell-free extracts. Two a...

The biosynthetic gene cluster for bluensomycin, a member of the aminoglycoside family of antibiotics, was isolated and characterized from the bluensomycin producing strain, Streptomyces bluensis ATCC27420. PCR primers were designed specifically to amplify a segment of the dTDP–glucose synthase ...

The objective of this study undertaken was to acquire maximum information by the shake flask studies for Ascomycin production with Streptomyces hygroscopicus subsp. ascomyceticus ATCC 14891. The morphological observation of culture on plate media was carried out visually and under stereo microsc...

BackgroundPatients with high-risk gestational trophoblastic neoplasia (GTN) need multi-agent chemotherapy to be cured. The most common regimen is etoposide (E), methotrexate (M) and actinomycin D (A), alternating weekly with cyclophosphamide (C) plus vincristine (O) (EMA/CO). Cisplatin (P) is a ...

A comprehensive study of the coordination ability of the Ag(I) ion with biologically oriented synthetic derivatives of naturally occurring molecular targets of antimycins and actinomycins, with potential anticancer activity, is presented. The physical properties and molecular structures of the i...

The high mobility group box 1 protein has been identified as a key player in chromatin homeostasis including transcription regulation, recombination, repair, and chromatin remodeling. Emerging findings indicate HMGB1 protein over expression in nearly all types of human cancers and inflammatory d...

Actinomycetes are main producers of antibiotics and targeted screening could improve the efficiency of discovering new drugs. This study describes, for the first time, the isolation of endophytic actinomycetes from the macrofungus Ganoderma applanatum. To increase the efficiency of screening, no...

The novel bioactive actinobacterial strain GSBNT10 obtained from a Saharan soil, was taxonomically characterized using a polyphasic approach. 16S rRNA gene sequence analysis supported the classification of the isolate within the genus Streptomyces indicating it as a novel species. The major meta...

ObjectiveTo evaluate the impact of periodic shortage of actinomycin-d (Act-d) in the treatment of Brazilian patients with low-risk gestational trophoblastic neoplasia (GTN) after methotrexate and folinic acid rescue (MTX/FA) resistance, treated alternately with carboplatin or etoposide as a seco...

Edeine binds to 70-S ribosomes in the presence of 10 mM MgCl2. At a concentration of Mg2+ of 1 mM the binding is 30 times greater than at 10 mM. Edeine does not affect the formation of polysomes, although some binding of edeine to polysomes is observed. Edeine causes association of 30-S and 50-S...

Enzymes obtained from cells disrupted by lysozyme treatment were resolved into three complementary enzyme fractions by DEAE-cellulose-Sephadex G-200 chromatography. These enzymes of molecular weight 210 000 (A), 180 000 (B) and 100 000 (C) were responsible for the activation of the constituent a...

Publisher SummaryThis chapter discusses the assay and purification procedure of edeine synthetases. In assay method, binding of the amino acyl to the enzyme complex can be measured only if radioactive edeine constituent amino acids are available. Formation of edeine A and B is measured by incorp...

The antibiotic inhibitor edeine has been used to study various aspects of the initiation of protein synthesis in a rabbit reticulocyte cell-free system. Edeine prevents assembly of the 80S initiation complex while allowing accumulation of a 44S initiation intermediate. The complete 80S initiatio...