Edeine inhibits the synthesis of bacterial DNA as shown by the incorporation studies of [3H]thymidine into DNA of Escherichia coli 15 T−. It does not induce a release of [3H]thymidine from prelabeled DNA. Edeine does not bind to the DNA. DNA isolated from edeine treated Bacillus subtilis shows t...

1.1. Exogenous edeine inhibits the synthesis of DNA and protein, but not that of RNA, in extracts of edeine-producing Bacillus brevis Vm 4 cells. This is analogous to the effect of edeine on extracts obtained from edeine-sensitive cells.2.2. Producer cells, in contrast to sensitive ones, are not...

The crystal structures of the universal translation-initiation inhibitors edeine and pactamycin bound to ribosomal 30S subunit have revealed that edeine induces base pairing of G693:C795, residues that constitute the pactamycin binding site. Here, we show that base pair formation by addition of ...

SummaryEdeines are atypical cationic peptides produced by Brevibacillus brevis Vm4 with broad-spectrum antimicrobial activity. These linear nonribosomal peptides bind to the 30S ribosomal subunit and block t-RNA binding to the P-site. To identify the mechanism of high-level self-resistance in th...

Reactions of thermorubin (1), an antibiotic produced by Thermoactinomyces antibioticus, with conventional reagents such as CH3NH2, NaBH4, HCHO/R2NH were studied. Several derivatives were obtained whose structures were determined on the basis of chemical and spectroscopic data. Some compounds sho...

The antibiotic thermorubin specifically inhibits protein synthesis when added to growing cultures of Escherichia coli, while RNA and DNA synthesis are not affected. In vitro studies show that thermorubin is a specific inhibitor of initiation of protein synthesis directed by natural mRNA. The dru...

Thermorubin is a small-molecule inhibitor of bacterial protein synthesis, but relatively little is known about the molecular mechanism by which it blocks translation. The structure of the complex between thermorubin and the 70S ribosome from Thermus thermophilus reported here shows that thermoru...

In trifluoroethanol stendomycin exhibits a CD pattern in which the extrema and ratios of peak magnitudes are virtually identical to α-helical poly-D-glutamic acid in water at pH 3.9. The results are presented to stimulate interest in the conformation of stendomycin as the most decisive test of ...

A comparison has been made of interferon production in mice injected intravenously with Escherichia coli endotoxin or statolon. Similar to previous findings with endotoxin, doses of cycloheximide which effectively blocked protein synthesis did not prevent the appearance of circulating interferon...

Statolon has been shown to induce, both in mouse cell tissue culture and in the whole animal, a virus inhibitor that possesses the properties of interferon. Species specificity is observed with statolon-induced chick and mouse interferons.Interferon production induced by statolon in chick tissue...

In this work we investigate the surface properties and foamability of saponin and mixed saponin-chitosan solutions. These natural compounds are widely used in various cosmetic, pharmaceutical and food technologies because of their efficiency as bio-active components and their biodegradability.Th...

Elongation factor Tu (EF-Tu) ofStreptoverticillium mobaraense, which produces pulvomycin, has been prepared to 90% purity. The purified protein differs significantly from the analogous protein found inEscherichia coli in molecular weight and antibiotic sensitivity. EF-Tu migrates in sodium dodec...

A spermine-dependent, polyphenylalanine-synthesizing cell-free system having an optimum activity at 75–85°C, has been developed from the extremely thermoacidophilic archaebacterium Caldariella acidophila. The C. acidophila system is totally insensitive to the EF-Tu targeted antibiotics pulvomy...

The effect of pulvomycin on the biochemical and fluorescence spectroscopic properties of the archaeal elongation factor 1α from Sulfolobus solfataricus (SsEF-1α), the functional analog of eubacterial EF-Tu, was investigated. The antibiotic was able to reduce in vitro the rate of protein synthe...

The effects of Pangamic acid (B-15) ingestion was examined during acute submaximal exercise on eight volunteer subjects. During the experimental condition subjects orally ingested 0.40-g tablets, six per day, of an equal molar mixture of calcium gluconate and N,N-dimethylglycine for 12 weeks. Wh...

A simultaneous method for analyzing sodium iron chlorophyllin (SIC) and sodium copper chlorophyllin (SCC) using high-performance liquid chromatography was developed. This method employed an Inertsil ODS-2 column and diode array detection at 395 nm, using methanol–water (97:3 and 80:20, v/v) con...

Mitochondrial DNA (mtDNA) is an important contributor to the ATP-generating oxidative phosphorylation complex. Single nucleotide mutations in mitochondrial genes involved in ATP synthesis result in a broad range of diseases. Leber optic atrophy and Leigh's syndrome are two such diseases ari...

The recombinogenicity of damaged chromosomes in diploid Saccharomyces cerevisiae cells treated with bleomycin and structurally related phleomycin was measured, along with aneuploidy and mutation events. Phleomycin was substantially (up to 26-fold) more effective than bleomycin in producing genet...

Phleomycin is one of the anticancer glycopeptide antibiotics which cause DNA cleavage. It is commonly used as a copper(II) complex. Therefore, it is important to study the metal ion binding process and to define the coordination mode. In this paper, we describe the acid-base properties of phleom...

The structure of monazomycin was determined by degradative study in combination with biosynthetic means using 13C-NMR.