In the present work, a more simple and expedite functionalization method was used envisaging the preparation of functionalized alumina monoliths for effective purification of immunoglobulins. Single stage sol-gel synthesis method was used for the silanization of alumina monoliths with (3-Aminopr...

Treatment of alkyne–Co2(CO)6 complexes possessing a methylidene moiety as well as a terminal hydroxy functionality with SnCl4 at 0°C effected tandem ring-closure reactions resulting in the formation of 1-oxaspiro[4.4]nonane, 1-oxaspiro[4.5]decane, 6-oxaspiro[4.5]decane, and 1-oxaspiro[5.5]unde...

The conjugate additions of a variety of organocopper reagents or dimethyl malonate anion to a spirocyclic cyclopent-2-enone connecting a 1,2:5,6-di-O-isopropylidene-α-d-glucofuranosyl ring as a constituent of the spiro structure, namely (1S,3R,4R,5R)-3,4-(isopropylidenedioxy)-1-[(1R)-1,2-(isopr...

A system for the synthesis of α-alkylidene-γ-butyrolactones via a one-pot C–H insertion/olefination sequence is described. The process is based on the rhodium catalysed C–H insertion reaction of α-diazo-α-(diethoxyphosphoryl)acetates. The mild reaction conditions, operational simplicity an...

A new spiro-β-methylene-γ-lactone annulation reaction is addressed for the synthesis of 4-methylene-2-oxaspiro[4.5]decan-1-one and its derivatives. 2-Cyclohexylidene-3-trimethylsilylprop-1-yl pivalate, obtained from cyclohexanone, was hydroxymethylated followed by desilylation to afford 2-[(1-...

The total synthesis of l- (or 1S,2R,3S,5S)-bicarbocyclic nucleosides (8–15) has been accomplished. The key intermediate 3 was synthesized in two steps from the known chiral compound 1 through a stereoselective cyclopropanation under Furukawa conditions and its X-ray structure has been determine...

The eastern part of the Guiana Shield, northern Amazonian Craton, in South America, represents a large orogenic belt developed during the Transamazonian orogenic cycle (2.26–1.95 Ga), which consists of extensive areas of Paleoproterozoic crust and two major Archean terranes: the Imataca Block, ...

A simple and sensitive electrochemical biosensor was developed for microRNA-196a detection, which is of important diagnostic significance for pancreatic cancer. It was based on cyclic enzymatic signal amplification (CESA) and template-free DNA extension reaction. In the presence of microRNA-196a...

Ascorbic acid (vitamin C) plays a significant role in the prevention of oxidative stress. In this process, ascorbate is oxidized to dehydroascorbate (DHA). We have investigated the impact of DHA on peptide/protein intramolecular disulfide formation as well as S-glutathionylation and S-homocystei...

N-3 fatty acids, including docosahexaenoic acid (DHA), have a beneficial effect in both pain and psychiatric disorders. In fact, we previously reported that stress-induced pain prolongation might be mediated through the suppression of the G-protein coupled-receptor 40/free fatty acid receptor 1 ...

An unified approach to the construction of the seven carbon atom framework of the title compounds, having a γ-oxoacrylate moiety as common structural feature, utilizing an intermolecular [3+2] cycloaddition of the nitrile oxide generated from a malic acid derived C-4 aldehyde fragment with meth...

Publisher SummaryCyclic ADP-ribose (cADPR) serves as a second messenger for Ca2+ mobilization from the endoplasmic reticulum via the ryanodine-like receptor (RyR), and this cADPR-mediated Ca2+ release from the RyR is activated by Ca2+/calmodulin-dependent protein kinase II. Cyclic ADP-ribose als...

Publisher SummaryCyclic ADP-ribose (cADPR) is a Ca2+-mobilizing cyclic nucleotide that functions as an endogenous modulator of the Ca2+-induced Ca2+ release mechanism in cells. Its synthesizing enzyme, ADP-ribosylcyclase, is widely distributed among animal tissues and is particularly abundant in...

irgA, a virulence gene in Vibrio cholerae, encodes a 77 kDa outer membrane protein. irgA expression is activated by irgB, which encodes a LysR-type transcription factor and is divergently transcribed from a promoter overlapping that of irgA. Expression of irgA and irgB is repressed by iron and F...

Maintaining a stable and balanced histone pool is of paramount importance for genome stability and fine regulation of DNA replication and transcription. This involves a complex regulatory machinery, exploiting transcription factors as well as histone chaperones, chromatin remodelers and modifier...

Chicken anemia virus (CAV) is a small, single-stranded DNA virus of Anelloviridae family. Its genome segments encode three proteins, VP1, VP2, and VP3. This study identified an interaction between VP2 and VP3 and mapped the interaction domains. Through the yeast two-hybrid (Y2H) system, VP2 was ...

To map the epitopes of VP2 protein of chicken anemia virus (CAV), VP2 was expressed as a fusion protein in Escherichia coli BL21 (DE3). The Western blot demonstrated that recombinant VP2 protein could be recognized by sera of chickens infected with CAV. Female BALB/c mice were immunized with pur...

Chicken anaemia virus (CAV) is an important poultry pathogen. CAV infection can lead to severe immunosuppression and mortality in birds. The viral proteins (VP1, VP2 and VP3) are major protective immunogens that determine the pathotype of CAV strains. The factors influencing the synonymous codon...

Background and study aimEvidence shows that vitamin D and cyclooxygenase type 2 (COX-2) might play role in aetiology/progression of cancer. It is suggested that antitumour effect of vitamin D depends on vitamin D-receptor (VDR) expression. Aim of the study was to determine vitamin D and polyunsa...

ContextThe aetiology and pathogenesis polycystic ovary syndrome (PCOS) remain uncertain and thus the relative studies are still crucial.