Background: Two deoxysugar glycosyltransferases (GTs), UrdGT1b and UrdGT1c, involved in urdamycin biosynthesis share 91% identical amino acids. However, the two GTs show different specificities for both nucleotide sugar and acceptor substrate. Generally, it is proposed that GTs are two-domain pr...

In our previous studies, the production of four bioactive molecules by Streptomyces sp. PAL114 in complex ISP2 broth medium has been described. Three of these molecules belong to the angucycline family. In this study, two novel antibiotics belonging to the same family were produced by strain PAL...

Background: Streptomyces fradiae is the principal producer of urdamycin A. The antibiotic consists of a polyketide-derived aglycone, which is glycosylated with four sugar components, 2× D-olivose (first and last sugar of a C-glycosidically bound trisaccharide chain at the 9-position), and 2× L...

Combinatorial biosynthesis is a promising technique used to provide modified natural products for drug development. To enzymatically bridge the gap between what is possible in aglycon biosynthesis and sugar derivatization, glycosyltransferases are the tools of choice. To overcome limitations set...

Background: Urdamycin A, the principle product of Streptomyces fradiae Tü2717, is an angucycline-type antibiotic. The polyketide-derived aglycone moiety is glycosylated at two positions, but only limited information is available about glycosyltransferases involved in urdamycin biosynthesis.Resu...

The chemo-enzymatic synthesis of (R)-5-hydroxymethyl-2-isopropyl-5-methylcyclopent-1-en-1-yl trifluoromethylsulfonate, a potential chiral building block for polycyclic terpenoids containing a five–membered ring having isopropyl and angular methyl substituents, such as erinacin A and dolatriol, ...

The complex of 2-(hydroxymethyl)-1-methyl-piperidine [2-(1-methyl-piperidine)-methanol] (MPMe) and p-hydroxybenzoic acid (HBA) crystallizes as a hydrate. HBA interacts through the OH···O and NH···O hydrogen bonds with MPMe. The water mediated hydrogen-bonded bridge molecules MPMe and HBA. ...

A potentiometric method was described for the determination of iron(III) in petroleum water samples using modified carbon paste and screen-printed sensors. These sensors were embodied with multi-walled carbon nanotubes (MWCNTs) since it improved the performance of the sensors in presence of (met...

The vesamicol analogue (±)-trans-2-Hydroxy-3-{4-(3-iodophenyl)piperidyl}-1,2,3,4-tetrahydronaphthalene (3′-IBVM), a potent ligand for the vesicular acetylcholine transporter (VAChT), was evaluated as a potential radiotracer for studying VAChT density in vivo. In radioligand binding experiments...

The removal of chloride from the zinc electrolyte produced during hydrometallurgical zinc production is challenging. The ion-exchange method is a promising way to remove chloride if the resin washing wastewater can be recycled. This paper focuses on chloride removal from resin washing wastewater...

Inefficient separation of inorganic salts and organic matters in crystallization mother liquor is still a problem to industrial wastewater treatment since the high salinity significantly impedes organic pollutants degradation by oxidation or incineration. In the study, acidification combined ele...

Ternary mutual diffusion coefficients measured by the Taylor dispersion method are reported for two aqueous systems, that is, DL-2-aminobutyric acid (AABA) + sodium chloride, and DL-4-aminobutyric acid (GABA) + sodium chloride at 298.15 K, and at different carrier concentrations (from 0 to 0.010...

The objective of the study was to synthesize deep eutectic solvents (DES) using choline chloride and citric acid monohydrate at different molar ratios (i.e. DES 3:1, 2:1, 1:1, 1:2 and 1:3) followed by physicochemical characterization. The DES produced were clear and homogenized liquids under a m...

The interfacial hydrolysis of trimesolyl chloride (TMC) is studied in this work. TMC, together with meta-phenylene diamine (mPDA), forms the pair of monomers commercially used extensively in the preparation of thin film composite membranes for reverse osmosis. The interfacial hydrolysis of TMC i...

Momordica charantia L. has been widely used in different countries as a functional food source, particularly as a remedy for type 2 diabetes. In the present study, 21 cucurbitane-type triterpenoids, including two new compounds named charantoside IX and X (1−2), were isolated from the extract of ...

2-[18F]Fluoro-l-tyrosine is a fluorine labelled amino acid, known to be incorporated into newly synthesised proteins, rendering it a potentially suitable tracer to image protein metabolism in vivo using positron emission tomography. For the electrophilic preparation of 2-[18F]fluoro-l-tyrosine t...

A practical procedure to regioselectively install a methyl group and a phenolic hydroxyl group onto l-tyrosine was developed. By using this approach, protected l-3-hydroxy-4-methoxy-5-methyl-phenylalanine and l-3-hydroxy-4-methoxy-5-methyl-phenylalanol, which are utilized in efficient syntheses ...

A novel and general approach has been developed to prepare l-tyrosine-containing porphyrins. The key intermediates, 2-tert-butoxycarbonylamino-3-(3-formyl-4-hexyloxy-phenyl)-propionic acid hexyl ester and 2-tert-butoxycarbonylamino-3-(3-formyl-4-methoxy-phenyl)-propionic acid methyl ester, were ...

A new strategy for the synthesis of l-3,4,5-trioxygenated phenylalanine derivatives from l-tyrosine is developed for the first time. The approach, featuring the transformation of aryl diiodide to bis-phenol via a one-pot procedure including lithiation, boronation, and oxidation, is highly practi...

A new reagent, 3-azaspiro[5,5]undecan-2,4-dioxo-3-yl diphenyl phosphate (ASUD-diphenyl phosphate) is described for the synthesis of N-protected amino acid-ASUD esters which are active esters useful in the synthesis of peptides. This compound was synthesized by reacting N-hydroxy-3-azaspiro[5,5]u...