Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants. In this study, chitosan modified carbon microcoils were designed and prepared for the selective separation of aristolochic acid I from me...

Phytochemical investigation on Aristolochia moupinensis and Aristolochia cathcartii resulted in the isolation of twelve aristolactam-type alkaloids and six aristolochic acid derivatives. Their structures were identified as aristololactam BII, 7-methoxyl-aristololactam IV, aristololactam IV, aris...

A novel fluorescent graft conjugated polymer (poly (2, 5-bis (Polyethylene glycol oxybutyrate)-1, 4-phenylethynylene-alt-1, 4-phenyleneethynylene, PPE-OB-PEG) has been designed and synthesized for the determination of aristolochic acid (AA). The detection conditions and detection characters of P...

In this work, we presented the preparation of magnetic carbon nanotubes (MCNTs) functionalized with molecularly imprinted polymers (MIPs) for effective removal of aristolochic acid I (AAI) in traditional Chinese medicine (TCM). [email protected] was obtained via a facile and environmental friend...

Previous studies have reported that the complement system is unconventionally activated in many kinds of glomerulonephritis. Multiple complement components participate in the pathogenic process by triggering immune response or other intracellular signaling pathways. Here, we have investigated th...

A convenient, and efficient multi-phase extraction method was used to isolate aristolochic acid from real samples. To increase the efficiency, a new dual ionic liquid-immobilized silica was used as a sorbent and the effects of the adsorption conditions were investigated. [email protected] in a m...

BackgroundAristolochic acid nephropathy (AAN) is a chronic, progressive interstitial nephritis. To date, treatment strategies remain limited. Mounting evidence shows that relaxin (RLX) possesses powerful anti-fibrotic and anti-apoptotic characteristics, therefore, the present study aimed to inve...

Ab initio molecular orbital calculations at the QCISD(T) / 6-311 + + G(d,P) / / MP2 / 6-31G(d,p) + ZPE level show that the suprafacial 1,3-hydrogen shifts in the 1-phosphapropene-vinylphosphine (H3CCHPH⇌H2CCHPH2) and degenerate 1,3-diphosphapropene (H2PCHPH⇌HPCHPH2) rearrangement...

The microscopic distribution of ω3 (peripheral type benzodiazepine) binding sites in sections from normal and ischaemic rat brain has been determined by emulsion autoradiography with the photoaffinity ligand [3H]PK 14105. In the normal rat brain, high densities of ω3 sites were present in the ...

To examine the relationship between PKBS, a 17-kD protein covalently photolabeled by [3H]PK 14105, and its association with peripheral-type benzodiazepine binding sites, rat adrenal mitochondrial fractions were photolabeled with [3H]PK 14105, solubilized in digitonin, and subjected to anion-exch...

A method has been developed for labelling PK 14105 [N-methyl-N-(1-methyl-propyl)-1(2-fluoro-5-nitro- phenyl)isoquinoline-3-carboxamide], a ligand that has high affinity and selectivity for peripheral type benzodiazepine binding sites (PBBS), with NCA fluorine-18 (t12 = 109.8 min, β+ = 96.9%). T...

The peripheral-type benzodiazepine receptor in rat kidney has been identified by photoaffinity labeling. PK 14105, a derivative of the selective peripheral-type ligand PK 11195, was used to covalently label peripheral-type benzodiazepine receptors. In the absence of UV light PK 14105 demonstrate...

Tamoxifen is a selective estrogen receptor modulator widely used in oncology and reproductive endocrinology. In order to decrease its non-desirable effects and elucidate mechanisms of action, permanently charged tamoxifen derivatives (PCTDs) have been reported. Whether PCTDs have genomic effects...

We have compared the cell and tissue selective estrogenic and antiestrogenic activities of tamoxifen, raloxifene, ICI 164,384 and a permanently ionized derivative of tamoxifen — tamoxifen methiodide (TMI). This non-steroidal antiestrogen has limited ability to cross the blood brain barrier and ...

The anticancer action of a permanently charged tamoxifen derivative, tamoxifen methiodide (TMI), was assessed in a model of breast cancer. In addition, analysis of MCF-7 cells in cultures was performed to consider the anticancer mechanism of TMI. Nude mice were implanted with MCF-7 human cancer ...

[15-13C2H3]-dihydro-epi-deoxyarteannuin B (4a) has been fed to intact Artemisia annua plants via the root and three labeled metabolites (17a–19a) have been identified by 1D- and 2D-NMR spectroscopies. The in vivo transformations of 4a in A. annua are proposed to involve enzymatically-mediated p...

A rapid high-pressure liquid chromatography (HPLC) tandem mass spectrometry (TQD) method for the determination of artemisinin, 9-epi-artemisinin, artemisitene, dihydroartemisinic acid, artemisinic acid and arteannuin B in Artemisia annua extracts is described. Detection and quantification of 9-e...

A series of the titled compounds were synthesized and tested for antibacterial activities in comparison with typical fluoroquinolones. (S)-3-Aminomethyl-3-fluoromethyl derivative (Y-688) was confirmed to be optimal because of being most active especially against Gram-positive bacteria including ...

1-(2-Furyl)ethanol 1 was resolved by irreversible transesterification with vinyl acetate using Lipozyme IM or Porcine Pancreas Lipase (PPL) in an organic solvent. (S)-alcohol (99% e.e.) was obtained in 80–85% yield using Lipozyme IM in carbon tetrachloride while (R)-1-(2-furyl)ethyl acetate (96...

Binding of the photosensitizer etiopurpurin (ET2) to human plasma was assessed, using conditions that would yield a high percentage of ET2 in the form of LDL-bound monomers which may favor photosensitizer tumor localization. Two delivery systems, Cremophor EL (CRM) and dimethyl sulphoxide (DMSO)...