In this work amine functionalized silicon nanoparticles colloid is utilized to prepare a fluorescent probe for detecting low concentration levels of hypochlorite in water. The sensor is prepared by adding organosilane in silicon nanoparticles colloidal solution which freshly synthesized by laser...

Reported here is a novel ratiometric and colorimetric fluorescent probe 1 for hypochlorite based on cyanobiphenyl and diaminomaleonitrile. This probe 1 was designed based on the mechanism that ClO− selectively cleaved the hydrazone bond (-C=N-) in this probe and released the fluorophore, 3`-form...

The objective of this study was to evaluate the cytotoxic effect of different sodium hypochlorite concentrations, using apical root cells of P. sativum as a bioindicator. Initially, the seeds of P. sativum were exposed to different concentrations of sodium hypochlorite (0.1, 0.2, 0.4, 0.8, 1.6, ...

A new fluorescence probe based on phenothiazine-coumarin platform was rationally designed and developed for the detection of hypochlorite in a ratiometric manner in aqueous solution and living cells. The probe possessed a large pseudo Stokes shift (209 nm) and lager gap between two emissions (46...

Hypochlorous acid is present in several biological systems and plays a pivotal role in bioprocesses. The acid also finds application in industrial and synthetic spheres. The ecological toxicity of elevated hypochlorite concentrations in aqueous media has been thoroughly documented. As a conseque...

The absorption of glycyl-proline, glycine, and ethanol through the intestinal wall was studied in vitro by an everted sac technique in rats infected with the coccidial parasite Eimeria nieschulzi which causes damage and atrophy to the intestinal villi. The absorption of the dipeptide and of the ...

Renal brush border vesicles prepared from rabbit were shown to transport intact glycyl-L-proline into the intravesicular space by a Na+-independent, carrier-mediated process. The kinetics of hydrolysis of glycyl-L-proline by the vesicles showed that this dipeptide was extremely resistant to hydr...

This study characterizes the transport of [1-14 C]glycyl-l-proline into purified brush border membrane vesicles prepared from human small intestine. Time-course uptake curves of glycyl-l-proline were similar under sodium thiocyanate or potassium thiocyanate gradient conditions (extravesicular > ...

Optical resolution by micellar electrokinetic chromatography with sodium N-tetradecanoyl-l-glutamate (STGlu) was investigated. Similar to the case when using sodium N-dodecanoyl-l-valinate (SDVal), sodium N-dodecanoyl-l-glutamate (SDGlu) and N-dodecanoyl-l-serine (DSer), addition of sodium dodec...

Optical resolution by micellar electrokinetic chromatography with N-dodecanoyl-l-serine (DSer) was investigated. Similarly to the use of sodium N-dodecanoyl-l-valinate or sodium N-dodecanoyl-l-glutamate, addition of sodium dodecyl sulfate (SDS), urea and organic modifiers such as methanol and 2-...

The previously reported genetic polymorphisms of the stearoyl-CoA desaturase (SCD1) and sterol regulatory element binding protein-1 (SREBP-1) genes were investigated in Fleckvieh bulls using the PCR-RFLP and AS-PCR methods, respectively. The genomic DNA was obtained from a total of 370 bulls. Th...

The stearoyl-coenzyme A desaturase system of L-M cells, grown as monolayers, was examined in microsomal membranes that contained 8.2% phosphatidylisopropylethanolamine, an unnatural phospholipid analog. Desaturation of both [1-14C]stearic acid by whole cells and [1-14C]stearoyl-coenzyme A by cel...

The mouse genome contains two stearoyl-CoA desaturase (SCD) structural genes (SCD1 and SCD2) that are expressed in a tissue-specific manner. Brain SCD2 mRNA levels are about 2-fold higher in pups nursed by mothers fed a control diet (5% corn oil (CO), (essential fatty acid (EFA) adequate)), comp...

We have recently reported that albumin, a serum protein present in the developing brain, stimulates the synthesis of oleic acid by cultured astrocytes by inducing stearoyl-CoA 9-desaturase, the rate-limiting enzyme in oleic acid synthesis, through activation of the sterol regulatory element-bind...

SummaryThe gut microbiota plays a key role in host metabolism. Toll-like receptor 5 (TLR5), a flagellin receptor, is required for gut microbiota homeostasis. Accordingly, TLR5-deficient (T5KO) mice are prone to develop microbiota-dependent metabolic syndrome. Here we observed that T5KO mice disp...

The effect of the stearoyl-CoA desaturase (SCD) and sterol regulatory binding transcription factor 1 (SREBF1) polymorphisms on the volatile compound profile of dry-cured hams from Italian protected denomination of origin (Prosciutto di Parma, Prosciutto di San Daniele and Prosciutto Toscano) was...

Deposition of oleate, stearate and palmitate at the later stages of seed development in Mahua (Madhuca longifolia (latifolia)), a tropical non-conventional oil seed plant, has been found to be the characteristic feature of the regulatory mechanism that produces the saturated fatty acid rich Mahu...

BackgroundLeucine stimulates Sirt1 and AMPK signaling in vitro and in vivo. Since metformin converges on the same pathway, we have tested the ability of leucine to amplify the effects of metformin on AMPK-mediated hepatic lipid metabolism in diet-induced-obese insulin-resistant mice.

Chiral separations by micellar electrokinetic chromatography with sodium N-dodecanoyl-l-valinate (SDVal) were investigated. Addition of sodium dodecyl sulphate (SDS), urea and methanol to SDVal micellar solutions improved the peak shapes and resolution and also changed the selectivity. Phenylthi...

Optical resolution by micellar electrokinetic chromatography with sodium N-dodecanoyl-l-glutamate (SDGlu) and with digitonin— sodium taurodeoxycholate (STDC) mixed micelles was investigated. Addition of sodium dodecyl sulphate, urea and methanol to SDGlu micellar solutions could give improved p...