Exposure of HT-29 colon carcinoma cells to 1-methyl-2-nitrosoimidazole (INO), a reductive metabolite of a model 2-nitroimidazole, induced concentration-dependent DNA damage detectable by conventional alkaline (single-strand breaks) and neutral (double-strand breaks) filter elution techiques. Elu...

In addition to their ability to radiosensitize, nitroimidazoles are selectively toxic toward hypoxic cells. Reduction of the nitro group is required to observe cytotoxicity. One of the reduction products believed to play a role in this cytotoxicity is the nitroso-derivative. One-methyl-2-nitroso...

We tested 1-methyl-2-nitrosoimidazole (IN% the two electron reduction product of 1-methyl-2-nitroimidazole (INO2) for its in vitro cytotoxicity and glutathione (GSH) depleting capabilities. The half life of INO was shown to be dependent on cell concentration above 105 cells/ml, decreasing with i...

Isradipine (PN 200-110) was given to eight young and nine elderly mildly hypertensive patients. Pharmacokinetic and pharmacodynamic studies were carried out after a single 5 mg dose and after three weeks dosing with 5 mg twice daily. Isradipine lowered blood pressure and was well tolerated. Plas...

Several pyridoxine compounds were found to be formed in a high yield in a growing culture of Sporobolomyces singularis containing lactose and pyridoxine. Three compounds, I, II and III, were isolated from cultured broth by Dowex 50W-X8 colum chromatography, paper chromatography, lyophilization, ...

Cathepsins B and X are lysosomal cysteine carboxypeptidases suggested as having a redundant role in cancer. They are involved in a number of processes leading to tumor progression but their role in the epithelial-mesenchymal transition (EMT) remains unknown. We have investigated the contribution...

Inflammation plays a central role in the processes associated with neurodegeneration. The inflammatory response is mediated by activated microglia that release inflammatory mediators to the neuronal environment. Microglia-derived lysosomal cathepsins, including cathepsin X, are increasingly reco...

Soil salinity is one of the major abiotic stresses that affect the crop productivity. Understanding the mechanisms by which plants transmit the signals to cellular machinery to trigger adaptive responses is essential to develop more stress tolerant crops. Barley (Hordeum vulgare L.) is an import...

Due to its importance as a cereal crop worldwide, high interest in the determination of factors influencing barley grain quality exists. This study focusses on the elucidation of protein networks affecting early grain developmental processes. NanoLC-based separation coupled to label-free MS dete...

The evolutionary mechanism where autopolyploid tolerate abiotic stresses better than its diploid ancestor is poorly known. Large-scale gene-expression changes were not observed in synthetic autopolyploids compared to their respective diploids. However, natural autopolyploids with longer evolutio...

The induction kinetics of both acyl carrier protein activity and lipid biosynthesis has been investigated during seed maturation in oil seed rape. Acyl carrier protein is induced prior to the onset of lipid biosynthesis and remains at a high level even after lipid biosynthesis has ceased. Acyl c...

Fusarium head blight (FHB) disease adversely affects grain quality and final yield in small-grain cereals including barley. In the present study, the effect of an artificial infection with Fusarium culmorum and an application of deoxynivalenol (DON) on barley spikes of cultivars Chevron and Peda...

The crystal structure of the fatty acid elongating enzyme β-ketoacyl [acyl carrier protein] synthase I (KAS I) from Escherichia coli has been determined to 2.3 Å resolution by molecular replacement using the recently solved crystal structure of KAS II as a search model. The crystal contains two...

Barley (Hordeum vulgare L.) is the fourth major cereal crop and shows high adaptive capabilities to diverse environments. Thus, it might represent a potential reservoir of novel genes to improve abiotic stress tolerance. In this study, a novel AP2/ERF transcription factor gene designated as HvSH...

Knowledge workers are highly regarded by employers for their innovation and creativity. Due to the intangible character of knowledge, managing knowledge workers requires different approaches, tools and methods than managing non-knowledge workers. There is a broadly shared idea that knowledge wor...

It is well-known that plants respond to the toxin cadmium (Cd), a widespread soil contaminant, by accumulating glutathione (GSH) and phytochelatin (PC). However, the factor(s) in the regulation of GSH and PC synthesis remain to be further identified. Here, we report that 14-3-3 κ and λ negativ...

Accurate quantum dynamics calculations have been carried out for the Na(3S) + DF(ν = 1–4, j = 0) → NaF + D, Na(3P) + DF(ν = 0, j = 0) → NaF + D/Na(3S) + DF and Na(3S) + HF(ν = 2–4, j = 2,4,6,9) → NaF + H reactions. The isotopic substitution decreases the reactivity remarkably in both Na(...

Inspired by marine compounds the derivatization of the natural pyrrolo[2,3-d]pyrimidine lead scaffold led to a series of novel compounds targeting the histamine H3 receptor. The focus was set on improved binding towards the receptor and to establish an initial structure-activity relationship for...

The new pyrimidine-pyrrole scaffolds (7a–7m) with substituted 1,2,3-traizole moiety were synthesized in good to mild yields and subjected for anti-cancer activity against melanoma and breast cancer cell lines using MTT assay. The compounds 7f and 7m exhibited highest anti-cancer activity agains...

AimsThis study investigated the hepatoprotective effects of both zafirlukast and vincamine and their possible role in the treatment of tamoxifen-induced liver injury in rats.