SummaryNusG/Spt5 proteins are the only transcription factors utilized by all cellular organisms. In enterobacteria, NusG antagonizes the transcription termination activity of Rho, a hexameric helicase, during the synthesis of ribosomal and actively translated mRNAs. Paradoxically, NusG helps Rho...
NusG/Spt5 is a transcription elongation factor that assists in DNA-templated RNA synthesis by cellular RNA polymerases (RNAP). The modular domain composition of NusG/Spt5 and the way it binds to RNAP are conserved in all three domains of life. NusG/Spt5 closes RNAP around the DNA binding channel...
In all domains of life, elongating RNA polymerases require the assistance of accessory factors to maintain their processivity and regulate their rate. Among these elongation factors, the Spt5/NusG factors stand out. Members of this protein family appear to be the only transcription accessory pro...
Rpb5 is a subunit shared by the three eukaryotic RNA polymerases although its role in transcription remains unclear. It has been proposed that it makes contact with the promoter DNA and to participate in the coordination of the opening/closing of the RNA polymerase II DNA cleft. Here, we report ...
Transcription coupled repair (TC-NER) is a subpathway of nucleotide excision repair triggered by stalling of RNA polymerase at DNA lesions. It has been suspected that transcriptional misincorporations of certain nucleotides opposite lesions that result in irreversible transcription stalling migh...
Elongation has emerged as a highly regulated step in the multistage process of transcription. Control of gene expression mediated through transcription elongation remains an unexplored area of study in Toxoplasma gondii where the demands of complex lifecycle necessitate a regulated transcription...
SummarySpt5 is an essential and conserved factor that functions in transcription and co-transcriptional processes. However, many aspects of the requirement for Spt5 in transcription are poorly understood. We have analyzed the consequences of Spt5 depletion in Schizosaccharomyces pombe using four...
SummaryThe MYC oncoprotein binds to promoter-proximal regions of virtually all transcribed genes and enhances RNA polymerase II (Pol II) function, but its precise mode of action is poorly understood. Using mass spectrometry of both MYC and Pol II complexes, we show here that MYC controls the ass...
Background: Staphylococcal epidermolytic toxins A and B (ETA and ETB) are responsible for the staphylococcal scalded skin syndrome of newborn and young infants; this condition can appear just a few hours after birth. These toxins cause the disorganization and disruption of the region between the...
The development of semiconductors capable of photocatalytic activity under visible light is very important, because such materials are potentially useful for performing photocatalytic conversion under solar illumination. However, these processes are frequently unselective because they involve th...
In this work, copper metallic nanoparticles were successfully immobilized on the surface of the amorphous carbon nitride using the pulse laser ablation in liquid (PLAL) technique in different times of laser irradiation (denoted as Cu-time/a-C3N4). This novel amorphous carbon nitride was fabricat...
The density of homogeneous phase fluid mixtures for various carbon dioxide (CO2)/organic solvent systems was measured in homogeneous phase. Solvents included cyclohexane (C6H12), methylcyclohexane (C6H11CH3), and ethylbenzene (C6H5C2H5). Density measurements were performed using a high-pressure ...
The C-scorpionate iron(II) complex [FeCl2(Tpm)] [Tpm =κ3-HC(C3H3N2)3] was immobilized on three nanoporous carbon supports to produce active, selective and recyclable catalysts for the oxidation of cyclohexane.The influence of the porous carbon supports on the performance of the heterogenized [F...
The behavioural sciences and individual psychology suggest that many factors should be examined to determine e-commerce adoption. Understanding the individual motives of e-commerce adoption is very important for creating better technologies. Countries with a rapid pace of technological advanceme...
Two prenylated flavonoid derivatives, 5-hydroxy-4′-methoxy-2″,2″-dimethylpyrano-(7,8:6″,5″)flavanone (1) and 5,4′-dihydroxy-[2″-(1-hydroxy-1-methylethyl)dihydrofurano]-(7,8:5″,4″)flavanone (2), were isolated from an ethyl acetate-soluble extract of the leaves of Macaranga conifera using...
This study was conducted to clarify the penetration properties of 4-[1-hydroxy-1-methylethyl]-2-propyl-1-[4-[2-[tetrazole-5-yl]phenyl]phenyl]methylimidazole-5-carboxylic acid monohydrate (CS-088), an ophthalmic agent, and the mechanism of the permeability-enhancing effect of EDTA and boric acid ...
Self-association properties of CS-088, an antiglaucoma ophthalmic agent, were investigated. Various analytical methods, such as surface tension measurement, demonstrated that CS-088 is a self-associating compound with critical micellar concentration (CMC) of approximately 10 mg/mL. Light scatter...
The aim of this study was to optimize the high-performance liquid chromatographic separation of (6S,4R)-(−)-6-hydroxy-4-(1-hydroxy-1-methylethyl)-1-cyclohexene-1-ethanol and its potential impurities to determine them in the active ingredient and in pharmaceutical formulations for purity and stab...
The microdissociation constants of 5-[1-hydroxy-2-[(methylethyl)amino]ethyl]-1-3-benzenediol have been determined by means of spectrophotometric and spectrofluorometric techniques. Experimental data were analyzed using the linear regression method. Values of 8.90, 10.0, 10.25, 9.15, and 12.5 hav...
The first α- and β-chiral water-soluble trialkylmonophosphines, 1 and 2, respectively, both with C3 symmetry, were synthesised from sodium phosphide and chiral mesylates, accessible from (S)-ethyl lactate. X-ray structures of a corresponding 2:1 gold(I) complex [12Au(I)]OTf and of a borane com...
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