A high-performance liquid chromatographic method without prederivatization was investigated for the direct determination of 4-nitrobenzoyl chloride and 4-nitrobenzoic acid. The separation was carried out on C3, C8 and C18 alkyl-bonded silica columns with cyclohexane-tetrahydrofuran as the eluent...

10 N-aminoacyl-O-4-nitrobenzoyl hydroxamates were investigated as potential inhibitors of aminopeptidases. While the metal-depending enzymes aminopeptidase M. aminopeptidase P and leucine aminopeptidase were inhibited reversibly by the compounds, the thiol enzyme cathepsin H was inhibited effici...

A simple and rapid high-performance liquid chromatographic method for monitoring the process of condensation between 2,4-diaminobenzenesulphonic acid and 4-nitrobenzoyl chloride was developed. The reaction products were separated on a reversed-phase Novapak C18 column using 5 mM tetrabutylammoni...

A catalytic synthetic method of 3,4-dihydroquinazolin-4-ones has been developed. When N-(2-nitrobenzoyl)amides were treated with carbon monoxide in the presence of a catalytic amount of selenium, reductive N-heterocyclization of N-(2-nitrobenzoyl)amide efficiently proceeded to give the correspon...

Rosiglitazone has shown promising anti-inflammation effect. To develop preferable anti-inflammatory agents, twenty-two rosiglitazone analogs were synthesized and their anti-inflammatory activity was evaluated. Among these compounds, 6i and 6k displayed excellent inhibitory activities on the prod...

An asymmetric approach to the mC7N epoxyquinone central unit of the manumycin antibiotics is described based on the enantioselective (89% ee) chiral phase transfer epoxidation of a substituted cyclohexenone. The chiral epoxide is employed in the first syntheses of the title compounds in enantiom...

The development of a general synthetic route to the manumycin family of antibiotics is described and exemplified by the first total synthesis of alisamycin in racemic form.

Carbon catabolite repression (CCR) is a very important mechanism employed in the utilization of carbon as an energy source, required for the regulation of growth, development and secondary metabolite production in fungi. Despite the wide study of this mechanism in fungi, little is known about th...

Rhizobium sp. RM and RS are Vigna radiata root nodule isolates with the ability to solubilize tricalcium phosphate and rock phosphate under 50 mM Tris-Cl buffering conditions. Rhizobium sp. RM and RS were unique as they could produce two different organic acids, gluconic acid and oxalic acid usi...

The catabolite control protein CcpA from Bacillus megaterium was overproduced as a fusion protein to a 6xhis affinity tag and purified to homogeneity. Polyclonal antibodies of high affinity and specificity were raised against the purified protein. The serum did not crossreact with purified Lac r...

Lignocellulose-utilizing biorefinery is a promising strategy for the sustainable production of value-added products such as bio-based polymers. Simultaneous consumption of glucose and xylose in Escherichia coli was achieved by overexpression of the gene encoding Mlc, a multiple regulator of gluc...

Catabolite repression collectively describes the reversible process by which during rapid catabolism of one carbon source, uptake and catabolism of poorer substrates is prevented. The common logic of the global sensory and regulatory mechanisms involved, with a central role for protein kinases/p...

Carbon catabolite repression refers to the preference of microbes to metabolize certain growth substrates over others in response to a variety of regulatory mechanisms. Such preferences are important for the fitness of organisms in their natural environments, but may hinder their performance as ...

In Bacillus subtilis, carbon catabolite control is mediated by the regulatory protein CcpA. In addition to loss of catabolite repression, ccpA mutants exhibit a severe growth defect. They are not able to grow with glucose and ammonium as single sources of carbon and nitrogen, respectively. Only ...

Poly(γ-glutamic acid) (PGA) is a polymer composed of l- and/or d-glutamic acids that is produced by Bacillus sp. Because the polymer has various features as water soluble, edible, non-toxic and so on, it has attracted attention as a candidate for many applications such as foods, cosmetics and s...

Transcriptional regulation in response to the preferred sugars is a widespread phenomenon in living organisms. In Escherichia coli, carbon catabolite repression of the lactose operon is relieved in the absence of glucose in the culture medium, signaled by an increase of intracellular cyclic aden...

Vinyl ester resins (VERs) are used in a variety of applications. However, alternative feedstocks and molecular structures have been recently investigated due to dwindling petroleum reserves and risks associated with bisphenol A (BPA), a leading petroleum-derived precursor for VERs, as well as en...

This paper presents a DFT quantum chemical investigation of the molecular conformation, NMR chemical shifts and vibrational transitions of N-(2-methylphenyl)methanesulfonamide and N-(3-methylphenyl)methanesulfonamide (C8H11NO2S) employing B3LYP exchange correlation. The vibrational wavenumbers w...

Treatment of N-t-butylbenzenesulfonamide with an excess of BuLi, followed by the reaction with methyl 2-(4-methylphenyl)propanoate, gave the corresponding 2-carboxybenzenesulfonamide, which underwent a sequence of consecutive N-deprotective cyclization process mediated by TMSCl–NaI–MeCN reagen...

The activity coefficients at infinite dilution, γ13∞ and gas–liquid partition coefficients, KL for 62 solutes: alkanes, alkenes, alkynes, cycloalkanes, aromatic hydrocarbons, alcohols, thiophene, ethers, ketones, esters, 1-nitropropane, butanal, acetonitrile, acetic acid and water in the ioni...