Bioimprinting is a technique in which an aqueous solution of a protein molecule along with the imprint molecule is dried to remove bulk water. Subtilisin was dissolved in an aqueous buffer with a substrate analog and precipitated with the substrate alcohol to obtain a dually bioimprinted enzyme ...

Proprotein convertase subtilisin/kexin type 9 inhibitors serve as a valuable addition to the armamentarium of lipid-lowering agents and have promising potential. By inhibiting the proprotein convertase subtilisin/kexin type 9 enzyme, this novel molecule leads to increased low-density lipoprotein...

In the present work, subtilisin gene from Bacillus subtilis PTTC 1023 was synthesized, cloned into the vector pD441-NH and expressed in E. coli BL21 (DE3). Recombinant subtilisin was purified in a single-step procedure by affinity chromatography. The molecular mass of the purified protein was de...

Preparation of the gold core and silver shell NP (AuAgNP) is challenging because of the facile oxidation of silver. Here such a NP is carefully synthesized and conjugated with subtilisin to arrive at a stable spherical material of 120–130 nm in diameter (AuAgSNP). A biomaterial prepared by immo...

BackgroundTrials have demonstrated that proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors are effective as an adjunct to statin therapy, but access and cost issues have limited their use in community practice.

The X-ray crystallographic structure of the mature form of subtilisin E-S7 (SES7) at 1.90 Å resolution is reported here. Structural comparisons between the previously reported propeptide-subtilisin E complex (1SCJ) and our mature form subtilisin E-S7 (6O44) provide insight into active site adjus...

Subtilisin-like proteases play crucial roles in host-pathogen interactions. Thus, protease inhibitors constitute important tools in the regulation of this interaction. CmPI-II is a Kazal proteinase inhibitor isolated from Cenchritis muricatus that inhibits subtilisin A, trypsin and elastases. Ba...

Subtilisins are a family of serine proteases used widely throughout the detergent, leather and food industries, with the identification and development of new enzymes holding much potential value. Thermus thermophilus HB8 was examined for serine proteases and found TTHA0724 gene. Sequence analys...

A new alkaloid, guan-fu base Y,has been isolated from Aconitum koreanum (Levl.)Raipaics and shown to be 2-acetoxy-14-hydroxyhetisine (2) from its nmr (1H, 13C) and mass spectra. A completed assignment of mmr peaka was made with the help of NOE and 2-D experiments which also suggest a revised str...

A gas chromatographic–electron capture detection (GC–ECD) method has been developed for determining Guan-Fu Base A (GFA), an experimental anti-arrhythmic, in human plasma. The method was based on one-step liquid–liquid extraction with toluene and chemical derivatization with pentafluoropropio...

The antibiotic griseoviridin binds to the larger subunit of Escherichia coli ribosomes blocking the interaction between the 3′ terminal end of peptidyl-tRNA and the donor site of the peptidyl transferase centre. Griseoviridin inhibits binding of chloramphenicol, thiamphenicol, lincomycin, eryth...

The solution conformation of griseoviridin, a broad spectrum antibiotic, has been determined by 1H-NMR in deuterated dimethylsulfoxide. The structural determination is based on experimental data of NOE constraints. Five structures were obtained from restrained molecular dynamics calculations, by...

We report for the first time the deposition pattern in the respiratory tract of a metered dose inhaler formulated with hydrofluoroalkane (HFA) 134a, in comparison with an inhaler containing chlorofluorocarbon (CFC) 12. The inhalers delivered 125 μg fusafungine per metered dose, and deposition w...

Haemophilus influenzae, a normal host of the nasopharynx of humans, may become a pathogen. The first step of infection is adherence to epithelial cells of the nasopharynx through glycopeptidic adhesins, or pili. Adherence to human epithelial cells in continuous lines, HeLa and Hep2, of 8 piliate...

Fusafungine is a peptide antibiotic mixture composed of several enniatins and active against Grampositive bacteria. Ionophoric properties of fusafungine have been studied in liposomes by measuring protoncation exchange by both fluorescence and 31P-nuclear magnetic resonance (NMR) and have been c...

Objective: To investigate the effect of fusafungine spray on pain and healing process after pediatric tonsillectomy. Methods: Sixty children with ages between 4 and 14 years underwent tonsillectomy or adenotonsillectomy. The patients were randomly divided into three groups and each group consist...

Publisher SummaryThis chapter presents a comprehensive review of chartreusin, its chemistry, biosynthesis, biological action and drug potential. Spurred on by its activity in mouse leukemias as well as in solid tumour test systems, the present interest in chartreusin is centered on its potential...

Bixin is the most used carothenoid in food industry. It is conventionally extracted from annatto seeds (Bixa orellana L.) by alkaline solutions or organic solvents, producing bixin with low purity and generating toxic waste. This study aimed to compare different clean technologies for extraction...

This work presents physicochemical, morphological, thermal, and pasting properties of a novel native starch obtained from defatted and depigmented annatto seeds for applications in food-related areas. At the end of the annatto seed processing chain, a non-conventional white starch was obtained f...

The effects of E-beam radiation on microbiota, colour, texture and sensorial properties of hard annatto-coloured cheese during storage at 4 and 14 °C were studied. Doses of up to 2 kGy provoked a decrease in the total bacterial number, although microbiota recovery to initial levels during stora...