Cadaverine is a useful chemical that can be produced by lysine decarboxylase up to molar concentration levels. To develop a convenient and reusable production process, we performed intracellular immobilization of lysine decarboxylase (CadA) using poly(3-hydroxybutyrate) (P(3HB)) and PhaP1 (P(3HB...

BackgroundPhasins are low molecular mass proteins that accumulate strongly in bacterial cells in response to the intracellular storage of polyhydroxyalkanoates (PHA). Although lacking catalytic activity, phasins are the major components of the surface of the PHA granules and could be potentially...

We have investigated whether parotin subunit (PS) and its partial synthetic peptide (P-10.2: TDDTAIVLLK), possess interleukin 1 (IL-1)-like activities, and act on cell lines other than lymphocytes. When Chang liver cells were cultured with P-10.2, PS or IL-1, P-10.2 and PS augmented the growth o...

The diabetic young rats, after a single dose of 0.30 mg/100 g of body weight of parotin returned to glycaemic levels near to normal. The hepatic glycogen of the same animals was increased compared to the controls. Two hypotheses were suggested: (a) parotin could act as a part of parotid gland se...

Bioassay data support the hypothesis that salivary glands participate in endocrine regulation of the development and maintenance of connective tissues. Resection of all three major salivary glands damages epiphyseal cartilages in young growing rats. A subunit obtained from parotin, an extract of...

Ito(1954) reported that parotin A, the protein fraction prepared from the salivary gland, caused a fall in serum calcium. This claim has been investigated using a preparation made from human parotid saliva and a bio assay in rabbits. Animals fasted but given no injection or other treatment showe...

Bioassay data support the hypothesis that the salivary gland participates in endocrine regulation of the development and maintenance of connective tissues. Epiphyseal cartilages become dystrophic in young growing rats after the resection of all major salivary glands. A subunit obtained from paro...

SummaryThe thiopeptide class of antibiotics targets the GTPase-associated center (GAC) of the ribosome to inhibit translation factor function. Using X-ray crystallography, we have determined the binding sites of thiostrepton (Thio), nosiheptide (Nosi), and micrococcin (Micro), on the Deinococcus...

The bacterial translational GTPases (initiation factor IF2, elongation factors EF-G and EF-Tu and release factor RF3) are involved in all stages of translation, and evidence indicates that they bind to overlapping sites on the ribosome, whereupon GTP hydrolysis is triggered. We provide evidence ...

Micrococcin-resistant mutants of Bacillus megaterium that carry mutations affecting ribosomal protein L11 have been characterised. The mutants fall into two groups. “L11-minus” strains containing an L11 gene with deletions, insertions or nonsense mutations which grow 2.5-fold slower than the w...

To enhance the production of micrococcin GO5, a bacteriocin produced by Micrococcus sp. GO5, cultivation conditions and medium composition were optimized. The optimal initial pH and temperature for bacteriocin production were 7.0–9.0 and 37 °C, respectively. Micrococcus sp. GO5 displayed the h...

Extensive NMR studies validate the 1978 Bycroft–Gowland hypothesis, hitherto unsupported by experimental evidence, regarding the constitution of micrococcin P1. Published synthetic work in the micrococcin area must be reinterpreted in light of these findings.

SummaryThe lack of proper treatment for serious infectious diseases due to the emergence of multidrug resistance reinforces the need for the discovery of novel antibiotics. This is particularly true for tuberculosis (TB) for which 3.7% of new cases and 20% of previously treated cases are estimat...

Hepatitis C virus (HCV) is considered a major public health concern worldwide. Despite recent advances in curing chronic hepatitis C, unmet medical needs still remain, especially due to the high economic burden of therapies. Accordingly, our study aimed to identify affordable novel HCV inhibitor...

The prevalence of NAFLD increases with age. As the main active ingredient of ginger, 6-gingerol significantly improves lipid metabolism abnormalities in adult rodents. However, few studies have reported its effect on age-related NAFLD. This study was to investigate the effects of 6-gingerol on a...

Presence of pungent gingerols in ginger oleoresin makes it an ideal natural flavoring candidate for the food industry. The study reports its incorporation for synergistic enhancement of flavor and nutraceutical portfolio of candied mango. The process is systematically optimized at bench-scale fo...

Subcritical water extraction is an eco-friendly method for the extraction of less polar compounds without the use of organic solvents. This study determined the extraction conditions that maximize the contents of 6-gingerol and 6-shogaol obtained from ginger pulp and peel. The highest yields of ...

Respiratory infection is a viral spreading disease and a common issue, particularly in kids. The treatments are available but have so many limitations because the drawback of this disease is more morbidity and mortality in the severely immune compromised. Even, the phyto-constituent antibacteria...

6-Gingerol, the major component of gingerols extracted from Zingiber officinale, has been shown to exhibit anti-inflammatory and antioxidant bioactivities. Since neuroinflammation plays an important role in neurodegenerative diseases, such as Alzheimer’s disease (AD), and astrocytes have been c...

Ichthyophthirius multifiliis is a ciliate parasite of freshwater fish with a global distribution and results in severe economic losses in aquaculture. The present study aimed to investigate the efficacy and antiparasitic mechanism of active compounds isolated from Zingiber officinale against I. ...