Four modified atrial natiuretic factor (ANF) analogs were designed and synthesized by the solid-phase method. Using human ANF-(99–126) as the reference compound the receptor binding affinity and biological activity of these analogs were examined by radioreceptor assay and in vivo experiments. P...

The effect of the adenosine receptor agonists, 2-chloro-N6-cyclopentyladenosine (CCPA) and 2-hexynyl-adenosine-5′-N-ethylcar☐amide (HENECA) on atrial natriuretic factor (ANF) release was investigated. The A1 adenosine receptor agonist CCPA markedly increased plasma ANF levels, following subcuta...

Adrenomedullin (ADM) is a new 52 amino acid peptide originally isolated from extracts of human pheochromocytoma. ADM's biologic properties are nearly identical to those of atrial natriuretic peptides. Thus, the 4 peptide hormones originating from amino acids 1–30 [long acting natriuretic p...

Background & AimsAtrial natriuretic factor (ANF) prevents increases in intracellular levels of cAMP that are induced by secretin in the exocrine pancreas. We investigated the contribution of cyclic adenosine monophosphate (cAMP) efflux to ANF inhibition of secretin signaling.

To determine the effects of atrial natriuretic factor (ANF) on renal dopamine (DA) metabolism, 3H-DA and 3H-l-DOPA uptake by renal tubular cells was measured in experiments carried out in vitro in Sprague–Dawley rats. The receptor type involved was also analyzed. The results indicate that ANF i...

We previously reported that intravenously administered atrial natriuretic factor (ANF) induced no salivation but enhanced agonist-evoked secretion in submandibular glands. The gene expression of ANF and natriuretic peptide receptors (NPR) was later reported in the glands. In the present study we...

Previous studies have established that hydride reduction of 3β-benzoyloxy-5α-cholest-8(14)-en-15-one yields two epimers (at C-15) of 5α-cholest-8(14)-en-3β,15-diol which were designated as diol A and B. Efficient enzymatic conversion of both compounds to cholesterol was observed. To determin...

5α-Cholest-8(14)-en-3β,9α-diol-15-one has been prepared by chemical synthesis in two steps from 5α-cholest-8(14)-en-3β-ol-15-one. 9α-Fluoro-5α-cholest-8(14)-en-3β-ol-15-one was prepared in 91% yield from the corresponding 9α-hydroxysterol. 9α-Fluoro-5α-cholest-8(14)-en-3,15-dione was ...

The enzymatic (rat liver mitochondria) conversion of 3β-hydroxy-5α-cholest-8(14)-en-15-one to 5α-cholest-8(14)-ene-3β,26-diol-15-one is described. The enzymatic product was judged, on the basis of 1H and 13C NMR studies, to be a 4:1 mixture of its 25R and 25S isomers. (25R)-5α-Cholest-8(14)...

Treatment of 3β-benzoyloxy-14α,15α-epoxy-5α-cholest-7-ene (I) with gaseous HCl in chloroform at −40°C gave, in 87% yield, 3β-benzoyloxy-7α,15β-dichloro-5α-cholest-8(14)-ene (III). Reduction of the latter compound with lithium aluminum hydride in ether at room temperature for 20 min gave...

5α-Cholest-8(14)-en-3β-ol-15-one is a potent inhibitor of sterol biosynthesis in mammalian cells in culture and has significant hypocholesterolemic activity upon oral administration to rodents and non-human primates. The conversion of the 15-ketosterol to cholesterol upon incubation with the 1...

A simple synthesis of the serum cholesterol depressant 3β-hydroxy-5α-cholest-8(14)-en-15-one (I) is described. The key intermediate, 3β-benzoyloxy-5α-cholesta-8,14-diene (III), was synthesized from cholesterol benzoate (II) with minimal purification of intermediates.

Reduction of 3β-benzoyloxy-cholest-8(14)-en 13-one with lithium aluminum hydride yielded two epimers (at C-15) of cholest-8(14)-en-3β,15-diol. Reduction with lithium aluminum tritide yielded [15α-3H]-cholest-8(14)-en-3β,15β-diol and [15β-3H]-cholest-8(14)-en-3β,155-diol. Both labeled comp...

A 4α-methylsterol was isolated from the seeds of Capsicum annuum and was identified as 4α-methyl-5α-cholest-8(14)-en-3β-ol. This seems to b

Reduction of 3β-benzoyloxy-14α,15α-epoxy-5α-cholest-7-ene with lithium in ethylenediamine gave 5α-cholest-8(14)-en-3β, 5α-diol in high yield. This procedure offers an alternate synthesis through the reductive rearrangement of an α,β-unsaturated steroidal epoxide.

We investigate ortho positronium (Ps) scattering from H(2s), H(3s) excited states of hydrogen using a model coupled-channel formalism that was previously found to yield correct binding and resonance energies for H(1s) targets [Phys. Rev. A 59 (1999) 2058]. Like Ho, and Yan and Ho, we find severa...

The electronic structures of zincite Pb-modulated bulk and thin-films were studied via X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT) techniques. Both XPS data and DFT-calculations allowed the derivation of two different Pb-embedding scenarios into the ZnO-hosts. Thes...

An efficient synthesis of 3-substituted indoles by a sequential approach involving gold(I) chloride catalyzed cycloisomerization/bis-addition and conjugate addition of 2-(alkynyl)anilines has been accomplished. A variety of 2-(alkynyl)anilines, aldehydes, isatins and nitroolefins undergo this ov...

The rates of elimination with sodium ethoxide in ethanol of the(2-phenyl-ethyl bromide)chromium tricarbonyl and of the(2-phenylethyl p-toluenesulphonate)chromium tricarbonyl weredetermined over a range of temperatures. The substrates gave thecomplexed styrene in 100% (Br) and 97% (OTs) yield. Fo...

Immobilization of organometallic complexes in thin supported films of ionic liquid has been used to generate a new class of hydroamination catalysts. High activities for the addition of aniline to styrene were obtained with a bi-functional system comprising [Pd(DPPF)](CF3CO2)2 and CF3SO3H immobi...