A new C-vinylpyrrole containing aroylhydrazone, ethyl 2-cyano-3-{5-[(4-nitro-benzoyl)-hydrazonomethyl]-1H-pyrrol-2-yl}-acrylate (ECNBHPA) derived from ethyl 2-cyano-3-(5-formyl-1H-pyrrol-2-yl)-acrylate and 4-nitro-benzohydrazide has been characterized by various spectroscopic techniques (1H NMR,...

After the addition of 5-aza-2′-deoxycytidine, a potent inhibitor of DNA methylation and S-phase-specific cytotoxic agent, metaphase chromosomes of Chinese hamster ovary (CHO) cells exhibited a highly decondensed and extended morphology (numerous “fragile sites”) at the first mitotic division....

In an attempt to understand the preferential action of 5-aza-2'-deoxycytidine against the lymphatic system, metabolic conversions of the tritiated drug in mouse liver and spleen in vivo and in vitro were followed. Simultaneously, the degradation of the drug resulting in the formation of N-a...

We investigated the cytotoxic activity and some aspects of the mode of action of 5-aza-2′-deoxycytidine (Aza-dC) in 21 primary cultures of leukemic cells freshly obtained from patients with chronic myeloid leukemia (CML) in blast crisis. The cytotoxic potency of Aza-dC was comparable or even gr...

Deoxycytidine kinase (dCK) and deoxycytidine deaminase (dCDA) are two key enzymes in the activation and inactivation, respectively, of deoxycytidine and its antiviral and anticancer analogues. One purpose of this study was to determine whether or not the deoxycytidine-converting activity of both...

5-Methyl-2′-deoxycytidine (5MedCyd) is a minor constituent of mammalian cell DNA. We report the production and characterization of highly specific rabbit anti-5MedCyd antiserum. The antiserum was suitable for the radioimmunological measurement of 5MedCyd. This simple radioimmunoassay was capabl...

Modification of DNA-cytosine by a 5-methyl group is thought to he an important mechanism which regulates the expression of eukaryotic genes. This modification takes place after semiconservative replication. There is very little evidence, if any. that 5MeCyt could be naturally incorporated into m...

Exposure of cultured L1210 cells to [2-14C]deoxycytidine and analysis of radioactivity incorporated into DNA-pyrimidines revealed that 2.7–5.5-fold more radioactivity is incorporated into DNA-thymine than into cytosine bases. Thus the pathway involving deamination of deoxycytidylate to deoxyuri...

The use of coastal forests as natural tsunami barrier has long been the source of controversies due to inconclusive understanding on the damping performance and contradictory field findings from the last tsunami events. After experiencing hundreds of casualties and severe destruction on the infr...

In order to develop high strength, high damping and low density Al matrix composites, the Al/Zn composite bar samples with Zn mass fraction of 10%-40% were prepared by powder extrusion. The tensile strength and damping properties of the samples are improved by controlling both the Zn/Al diffusio...

Coercivity, hardness and amplitude dependence of damping of electrochemically deposited nickel layers were measured directly after electrochemical deposition and subsequently after isochronal heat treatments at various annealing temperatures. All as-electrodeposited nickel samples have ultrafine...

Lower performance of multi-crystalline silicon solar cells is usually observed after long-term damp heat test at 85 °C/85% relative humidity. Performance degradation is known to result from the loss in fill factor (FF) due to high series resistance. The causes of the increase in the series resi...

Burns are associated with activation of the innate immunity that can contribute to complications. Damage-associated molecular patterns (DAMPs) released after tissue injury play a critical role in the activation of the innate immunity, which appears to be mediated via toll-like receptors (TLRs). ...

The amplitude-dependent damping of 304L austenitic stainless steel with prior fatigue loading is investigated using dynamic mechanical analysis (DMA). The results show the damping curves of the steel samples are associated with the prior fatigue state. There is a peak in the damping curve when t...

We systematically studied the effect of annealing on damping capacity of pre-deformed magnesium alloy and demonstrated the close relationship between damping capacity and twin boundary mobility. The damping capacity of pre-compressed AZ31 alloy can be improved by annealing for short period as a ...

The microstructure, stacking fault energy (SFE), tensile properties, fractured surface, and the damping capacity of the Fe–17Mn (wt.%) alloy with a dual phase structure of γ austenite and ε martensite were systematically investigated at different tool rotation speeds (i.e., 120, 280, and 440 ...

Resonance Raman spectra of proto-, meso-, and deuterohemes isolated in cytochrome b5 have been recorded and effects due to sidegroup substituents noted. A one-to-one correspondence can be made between the fundamental Raman bands of mesoheme and deuteroheme, enabling identification of inductive e...

The structure of horse methemoglobin reconstituted with deuteroheme, in which the heme vinyl substituents are replaced by hydrogens, has been compared with that of native horse methemoglobin by X-ray difference Fourier techniques. The tertiary structures of the two molecules are almost completel...

Deuterohemins III and XIII were coupled with apomyoglobin to examine the influence of the modified heme-globin contacts on the functions of the reconstituted holoproteins. Owing to the molecular symmetry of the prosthetic groups, the resultant proteins are free from the heme orientational disord...

The ternary phase equilibrium for 1-(3-nitrophenyl)ethanone and 1-(4-nitrophenyl)ethanone in methanol or n-propanol were determined at 298.15, 308.15 K and 318.15 K under atmospheric pressure in this work. Based on the measured mutual solubility data, the isothermal ternary phase diagrams were c...