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inquirysodium cholate
Conditions | Yield |
---|---|
In tetrahydrofuran |
Conditions | Yield |
---|---|
With disodium hydrogenphosphate; sodium hydroxide pH=8.3; |
sodium cholate
12α-hydroxy-3-oxochol-4-en-24-oic acid
Conditions | Yield |
---|---|
With Pseudomonas mendocina ECS10 In water at 28℃; for 24h; Microbiological reaction; | 95% |
Conditions | Yield |
---|---|
In dichloromethane; water for 48h; | 92% |
Conditions | Yield |
---|---|
With sulfuric acid for 3h; Heating; | 91% |
sodium cholate
1,3,5-tris(bromomethyl)-2,4,6-triethylbenzene
C87H138O12
Conditions | Yield |
---|---|
In N,N-dimethyl-formamide at 75 - 80℃; Inert atmosphere; | 87% |
1-naphthylmethyl 3α,12α-bis(chloroacetyloxy)-5β-cholan-24-oate
sodium cholate
Conditions | Yield |
---|---|
In N,N-dimethyl-formamide at 60℃; for 7h; | 86% |
Conditions | Yield |
---|---|
In toluene Inert atmosphere; Reflux; | 84.6% |
Conditions | Yield |
---|---|
In N,N-dimethyl-formamide at 75 - 80℃; Inert atmosphere; | 84% |
Conditions | Yield |
---|---|
In toluene Inert atmosphere; Reflux; | 83.9% |
sodium cholate
12β,17β-dihydroxypregna-1,4-dien-3-one-20-carboxylic acid
Conditions | Yield |
---|---|
With Pseudomonas fragi ECS9 In water at 28℃; for 48h; Microbiological reaction; | 80% |
Conditions | Yield |
---|---|
In toluene Inert atmosphere; Reflux; | 73.4% |
Conditions | Yield |
---|---|
With phosphate buffer; 3α-HSDH from Pseudomonas paucimobilis; sodium pyruvate; NAD In water at 22℃; for 48h; | 72% |
With DL-dithiothreitol; NAD In water; ethyl acetate for 40h; Ambient temperature; 3α-hydroxysteroid dehydrogenase, puryvate/lactic dehydrogenase, serum albumin, potassium phosphate buffer pH=8.5; Yield given; | |
With Bacillus mycoides B23 broth In water at 30℃; for 48h; | 91 % Chromat. |
1-naphthylmethyl 3α,7α,12α-tris(chloroacetyloxy)-5β-cholan-24-oate
sodium cholate
Conditions | Yield |
---|---|
In N,N-dimethyl-formamide at 60℃; | 66% |
sodium cholate
chloroformic acid ethyl ester
3α,12α,24-trihydroxy-5β-cholane
Conditions | Yield |
---|---|
Stage #1: sodium cholate; chloroformic acid ethyl ester In tetrahydrofuran at 50℃; for 20h; Inert atmosphere; Stage #2: With sodium tetrahydroborate In tetrahydrofuran; water at 0 - 20℃; for 3h; Inert atmosphere; | 63% |
sodium cholate
A
3-keto-12α-hydroxy-5β-cholan-24-oic acid
B
3,12-dioxo-7-deoxycholic acid
Conditions | Yield |
---|---|
With Rhodococcus erythropolis ECS25 In water at 28℃; for 24h; Microbiological reaction; | A 61% B 30% |
sodium cholate
Conditions | Yield |
---|---|
With Rhodococcus rubber for 24h; | A 55% B 20% |
sodium cholate
A
3α,6α-dihydroxy-7-oxo-5β-cholan-24-oic acid
Conditions | Yield |
---|---|
With Bacillus mycoides B23 broth In water at 32℃; for 24h; | A 34% B 50% |
Conditions | Yield |
---|---|
With 1-ethyl-(3-(3-dimethylamino)propyl)-carbodiimide hydrochloride; triethylamine In dichloromethane at 20℃; for 16h; Inert atmosphere; | 42% |
sodium cholate
A
3-keto-12α-hydroxy-5β-cholan-24-oic acid
B
12α-hydroxy-3-oxochol-4-en-24-oic acid
Conditions | Yield |
---|---|
With Pseudomonas prosekii ECS1 In water at 28℃; for 72h; Microbiological reaction; | A 20% B 22% |
methanol
sodium cholate
A
12α-hydroxypregn-4-en-3-one-20-carboxylic acid methyl ester
B
methyl 12α-hydroxy-3-oxo-5β-cholan-24-oate
C
(S)-2-((8R,9S,10R,12S,13S,14S,17R)-12-Hydroxy-10,13-dimethyl-3-oxo-6,7,8,9,10,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a]phenanthren-17-yl)-propionic acid methyl ester
D
methyl 12α-hydroxy-3-ketochola-1,4-dienoate
Conditions | Yield |
---|---|
With boron trifluoride 1.) water, 28 deg C, 16 h, Pseudomonas NCIB 10590, K2HPO4, KHPO4, KNO3, FeSO4*7H2O, ZnSO4*7H2O, MnSO4*4H2O, MgSO4*7H2O, pH=7, aeration; Yield given. Multistep reaction. Further byproducts given. Yields of byproduct given; |
Conditions | Yield |
---|---|
With isobutyl chloroformate 1) THF, -15 deg C, 8 min, 2) DMF, RT, 2 h; Yield given. Multistep reaction; |
sodium cholate
A
12β-hydroxy-1,4-androstadiene-3,17-dione
B
12β,17β-dihydroxypregna-1,4-dien-3-one-20-carboxylic acid
C
12β,17β-dihydroxyandrosta-1,4-dien-3-one
D
12α-hydroxyandrosta-1,4-dien-3,17-dione
Conditions | Yield |
---|---|
In water at 28℃; for 16h; Pseudomonas NCIB 10590, K2HPO4, KHPO4, KNO3, FeSO4*7H2O, ZnSO4*7H2O, MnSO4*4H2O, MgSO4*7H2O, pH=7, aeration; Further byproducts given; | A 7.6 g B 5 g C 150 mg D 30 mg |
sodium cholate
3,12β-dihydroxy-9,10-seco-1,3,5(10)-androstatriene-9,17-dione
Conditions | Yield |
---|---|
Pseudomonas putida ATCC 31752; |
sodium cholate
3α,6α-dihydroxy-7-oxo-5β-cholan-24-oic acid
Conditions | Yield |
---|---|
With Pseudomonas fluorescens BS7 broth In water at 30℃; for 48h; | 96 % Chromat. |
Conditions | Yield |
---|---|
With Acinetobacter calcoaceticus lwoffii BS11 broth In water at 30℃; for 48h; | 98 % Chromat. |
Conditions | Yield |
---|---|
With Pseudomonas fluorescens B25 broth In water at 30℃; for 48h; | 98 % Chromat. |
Conditions | Yield |
---|---|
With phosphate buffer pH=5.5; ion pair formation; |
sodium cholate
methyl 3α,12α-bis(1-imidazolylcarbonyloxy)-5β-cholan-24-oate
Conditions | Yield |
---|---|
Multi-step reaction with 2 steps 1: 91 percent / H2SO4 / 3 h / Heating 2: 97 percent / triethylamine / CH2Cl2 / 5 h / 40 °C View Scheme |
sodium cholate
methyl 3α,12α-bis(N,N-dimethylaminoethylcarbamoyloxy)-5β-cholan-24-oate
Conditions | Yield |
---|---|
Multi-step reaction with 3 steps 1: 91 percent / H2SO4 / 3 h / Heating 2: 97 percent / triethylamine / CH2Cl2 / 5 h / 40 °C 3: 66 percent / CH2Cl2 / 8 h / 25 °C View Scheme |
sodium cholate
Conditions | Yield |
---|---|
Multi-step reaction with 2 steps 1: 91 percent / H2SO4 / 3 h / Heating 2: 81 percent / pyridine / CHCl3 / 1 h / 25 °C View Scheme |
sodium cholate
methyl 3α,12α-bis(N-tert-butyloxycarbonylamidocapronoyloxy)-5β-cholan-24-oate
Conditions | Yield |
---|---|
Multi-step reaction with 2 steps 1: 91 percent / H2SO4 / 3 h / Heating 2: 64 percent / DCC; DMAP / CH2Cl2 / 23 h / 25 °C View Scheme |
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