Density functional calculations were performed on N,N′-bis(3′-carboxy,4′-aminophenyl)-1,4-quinonediimine, a carboxylic acid substituted aniline trimer. Results of the calculations were compared to experimental properties of the herein synthesized trimer, as well as to the properties of the an...

A new highly luminescent iridium complex, bis(2-phenylpyridine-N,C2′)[2-(2′-tosylaminophenyl)benzoxazole-N,N′]iridium(III) [Ir(ppy)2(TAPBO)] (TAPBO- 2-(2′-tosylaminophenyl)benzoxazole) has been synthesized and its molecular structure determined using single crystal X-ray diffraction analysis...

A photopolymer composition based on poly[N,N′-(1,3-phenylene)isophthalamide] and 3,3-bis(4-acrylamidophenyl)phthalide was developed and utilized for the formation of 3-dimensional structures using the laser stereolithography method with a high reproducibility and the absence of any autopolymeri...

The thermal reactions of ReH7(dppe) (dppe = Ph2PCH2CH2PPh2) with pyridine-2-carboxylic acid (Hpic), 1-isoquinolinecarboxylic acid (Hisoquin), 2-hydroxy-6-methylpyridine (Hmhp) and 2-mercaptoquinoline (Hmq) in refluxing benzene afforded the monohydridorhenium(III) species ReH(L)2(dppe) [L = pic (...

Car☐yarabinitol-1-phosphate (CA1P) is a naturally occurring inhibitor of ribulose-1,5-bisphosphate car☐ylase/oxygenase (rubisco) which appears in the leaves of some plants during darkness and occupies the ribulose-1,5-bisphosphate binding site. The involvement of the rubisco activase protein in ...

Ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) catalyzes the initial steps of photosynthetic carbon reduction and photorespiratory carbon oxidation cycles by combining CO2 and O2, respectively, with ribulose-1,5-bisphosphate. Many photosynthetic organisms have form I rubiscos comprise...

The level of 2-carboxyarabinitol (2-C-hydroxymethyl ribonic acid) was measured in leaves of numerous plants and its phylogenetic distribution then eval

Metabolism of 2-carboxyarabinitol 1-phosphate (CA 1-P), an endogenous inhibitor of ribulose bisphosphate carboxylase/oxygenase, occurs in the light. A soluble protein fraction which metabolized CA 1-P in the presence of NADPH was isolated from tobacco chloroplasts. A similar fraction from spinac...

In situ RuBisCO activity was analyzed in order to determine whether 2-carboxyarabinitol 1-phosphate (CA1P) interacts with the enzyme in dark-adapted leaves of Phaseolus vulgaris. Leaves ground to fine powder in liquid nitrogen were put directly into a reaction mixture containing a saturating con...

An enzyme has been identified in the stroma of tobacco chloroplasts that degrades the natural inhibitor of Rubisco, 2′-carboxyarabinitol 1-phosphate (2CA1P). The products of the catalysis, determined by HPLC and phosphate analysis are 2′-carboxyarabinitol and inorganic phosphate indicating tha...

A hybrid closed bomb-strand burner is used to measure the burning behavior of the titanium subhydride potassium perchlorate pyrotechnic with an equivalent hydrogen concentration of 1.65. This experimental facility allows for simultaneous measurement of the closed bomb pressure rise and pyrotechn...

We have previously reported that 4-methylumbelliferyl 6′-O-benzyl-β-lactoside (2) is a useful substrate for a fluorometric assay of ceramide glycanase (CGase) (L.-X. Wang, N.V. Pavlova, S.-C. Li, Y.-T. Li and Y.C. Lee, Glycoconjugate J., 13 (1996) 359–365). The introduction of a 6-O-benzyl gr...

The functional importance of glycolipids has emphasized the need for more sensitive methods of detection, characterization, and quantification than has often been possible using traditional thin-layer chromatographic techniques. We describe the use of ceramide glycanase and HPLC to identify and ...

SummaryA novel type of enzyme which hydrolyzes the linkage between the ceramide and the sugar chain in various glycosphingolipids has been found in the leech, Hirudomedicinalis. This enzyme releases the intact oligosaccharide from LacCer, GbOse3Cer, GbOse4Cer, GbOse5Cer, nLcOse4Cer, GM3, GM2, GM...

A bi-fluorescence labeled derivative suitable for analysis of ceramide glycanase activity was constructed from 4-pentenyl lactoside. Selective modification of the galactosyl residue was attained by formation of 4′,6′-naphthylmethylidene derivative, which was followed by regioselective reductiv...

Interest in cellular glycosphingolipid (GSL) function has necessitated the development of a rapid and sensitive method to both analyze and characterize the full complement of structures present in various cells and tissues. An optimized method to characterize oligosaccharides released from glyco...

Publisher SummaryCeramide glycanase (CGase) is an endoglycosidase that releases the intact glycan chain from various glycosphingolipids by cleaving the linkage between the ceramide and the glycan chain. This enzyme is found in the leech Macrobdella and in other annelids such as the European leec...

Publisher SummaryThis chapter describes various methods for preparing ceramide glycanases (cer-glycanase) from leech (Hirudo medicinalis) and earthworm (Lumbricus terrestris). These organisms are suitable for the structural analysis of ceramide glycans. Because cer-glycanase can release the inta...

An alkyl lactoside containing two different fluorescence probes as an energy donor and an energy acceptor was synthesized as a substrate for ceramide glycanase. n-Pentenyl β-lactoside was converted into its 4′,6′-O-(2-naphthylmethylidene) derivative with subsequent benzoylation of all remaini...

Publisher SummaryCeramide glycanase (CGase), the endoglycosidase that cleaves various glycosphingolipids in a one-step process liberating ceramide and the corresponding oligosaccharides, is characterized from different annelids, bacteria, and mollusk. The same CGase activity has also been detect...