We designed and tested three PilA-derived vaccine candidates in a chinchilla model of ascending nontypeable Haemophilus influenzae (NTHI)-induced otitis media (OM). Delivery of antiserum directed against each immunogen conferred varying degrees of protection. Presentation of a B-cell epitope der...

PilA is the response regulator of a two-component regulatory system that controls a number of genes in the pathogenic Neisseria. Previous work has shown thatNeisseria gonorrhoeae(GC) PilA binds DNA and also hydrolyzes GTP. Here, we report the cloning, sequencing, purification, and biochemical ch...

Geobacter sulfurreducens is a model organism for understanding the role of bacterial structures in extracellular electron transfer mechanism (EET). This kind of bacteria relies on different structures such as type IV pili and over 100 c-type cytochromes to perform EET towards soluble and insolub...

Pseudomonas aeruginosa type IV pili have an essential role in twitching motility, colonization and biofilm formation. In this study, we investigated the efficacy of intraperitoneal administration of rabbit anti-recombinant PilA (anti-r-PilA) immunoglobulin G (IgG) against P. aeruginosa infection...

This paper describes the synthesis and characterization of a nanocrystalline hydroxyapatite (HAp) bioceramics using dead biomass of Pila globosa shells. The high calcium carbonate content (94.76%) analyzed by inductively coupled plasma atomic emission spectroscopy confirmed the snail shell to be...

The lungs are a major site of Pseudomonas aeruginosa infection in patients with compromised immune systems. We have shown that a large number of cells of the P. aeruginosa wild-type PAO1 strain invade cultured human bronchial epithelial cells (BEAS-2B). In the present study, we investigated whet...

Bacterial fruit blotch (BFB) of cucurbits, caused by Acidovorax citrulli, is a major threat to commercial watermelon and melon production worldwide. At present, there are at least two genetically distinct sub-populations (group I and II) of A. citrulli that differ in host preference among cucurb...

Infections with Pseudomonas aeruginosa (P. aeruginosa) have been a long-standing challenge for clinical therapy, because of the complexity of pathogenesis and its widespread resistance to antibiotics. The main aim of the present study was to design and analyze a novel PilQ380–706-PilA fusion pr...

Previous studies demonstrated that the −82 to +87 nucleotides (nt) 5′-upstream region of the chicken apolipoprotein (apoAI) gene are necessary for maximum reporter chloramphenicol acetyl transferase (cat) gene activation in chicken hepatocarcinoma (LMH) cells [Bhattacharyya, N., Chattapadhyay, ...

The structure of the integral membrane protein OmpX from Escherichia coli reconstituted in 60 kDa DHPC micelles (OmpX/DHPC) was calculated from 526 NOE upper limit distance constraints. The structure determination was based on complete sequence-specific assignments for the amide protons and the ...

AimThe aim of the present study is to prepare and evaluate nanoparticles containing stavudine using chitosan as the polymer.

Despite continuous research and availability of 25 different active compounds for treating chronic HIV-1 infection, there is no absolute cure for this deadly disease. Primarily, the residual viremia remains hidden in latently infected reservoir sites and persistently release the viral RNA into t...

IntroductionIn this study, mechanical stress in the form of tooth movement was applied to osteoprotegerin-deficient knockout mice, which served as an animal model for juvenile Paget’s disease. To compare and evaluate bone turnover and response of the surrounding bony tissue, we administered rev...

We present new high precision Os isotope data obtained by solution-mode MC-ICP-MS for 4 different Os isotope reference materials and compare the data to that obtained by the N-TIMS method. Mass fractionation effects for MC-ICP-MS are evaluated and we demonstrate excellent adherence to the expone...

The metrological compatibility of n(234U)/n(238U), n(235U)/n(238U) and n(236U)/n(238U) isotope amount ratio measurement results obtained by gas source mass spectrometry (GSMS), thermal ionisation mass spectrometry (TIMS) and multi-collector inductively coupled plasma mass spectrometry (MC-ICPMS)...

The main geological feature of Central Cameroon is the wide spread occurrence of granitoids emplaced in close association with transcurrent regional shear zones. The basement of this vast domain is a Paleoproterozoic ortho-and para-derivative formation, which has been intensely reworked, togethe...

A Monte Carlo simulation with a coarse-grained (CGMC) model of protein involving a phenomenological residue–residue interaction potential, augmented by a Molecular Dynamics simulation is used to investigate local and global structures of a protein (hHv1) as a function of temperature. Data from ...

Laryngeal carcinoma is the second commonest and most aggressive cancer originating in the squamous cells of the laryngeal epithelium. Undesirable complications and resistance to chemotherapy are the common problems which necessitate the development of alternative anticancer agents for the manage...

Butafenacil is an herbicide that inhibits protoporphyrinogen oxidase (PPOX), an enzyme that catalyzes oxidation of protoporphyrinogen IX to protoporphyrin IX during chlorophyll and heme biosynthesis. Based on a high-content screen, we previously identified butafenacil as a potent inducer of anem...

Disorders of hemolysis reduce the exposure time of hemoglobin to glucose, resulting in a falsely low hemoglobin A1c level. This case report describes the unexpected diagnosis of glucose-6-phosphate dehydrogenase deficiency made during evaluation of discordant HbA1c and blood glucose measurements.