The density (ρ), viscosity (η) and conductivity (Ʌ) studies of Aspartic Acid and Glutamic Acid in aqueous solution of Dopamine hydrochloride were carried out at three different temperatures having an interval of 10 K under atmospheric pressure. The experimental data were methodically used to e...

As part of an investigation to generate optimized drug leads from marine natural pharmacophores for the treatment of neoplastic and infectious diseases, a series of novel isoaaptamine analogs were prepared by coupling acyl halides to the C9 position of isoaaptamine (2) isolated from the Aaptos s...

We analyzed the effects of all three marine alkaloids aaptamine, demethyloxyaaptamine and isoaaptamine in NT2-R, a cisplatin-resistant subline of the human embryonal carcinoma cell line NT2. All aaptamines were found to be equally effective in both cell lines, excluding cross-resistance between ...

Background: Many enzymes are active only in a dimeric form, including a variety of type II restriction endonucleases. Disruption of subunit interactions is therefore a potential method for multimeric enzyme inhibition. EcoRl is a homodimeric restriction endonuclease, the dimeric interface of whi...

The nucleotide sequence of a 1394 basepair (bp) DNA fragment containing the EcoRII restriction endonuclease (R.EcoRII) gene was determined. The endonuclease gene is 1206 bp in length (predicted 402 amino acids (aa) and Mr = 45 178) and is separated by 33 bp from the EcoRII modification methylase...

A new class II restriction endonuclease, AsnI, with a novel sequence specificity was isolated from the Gram-positive eubacterium Arthrobacter species, strain N-CM. AsnI recognizes the unambiguously defined palindromic hexanucleotide consisting of A- and T-residues. The novel enzyme in the prese...

SummaryThe structure of the rare-cutting restriction endonuclease NotI, which recognizes the 8 bp target 5′-GCGGCCGC-3′, has been solved with and without bound DNA. Because of its specificity (recognizing a site that occurs once per 65 kb), NotI is used to generate large genomic fragments and ...

Recently determined crystal structures of type II restriction endonucleases have produced a plethora of information on the basis for target site sequence selectivity. The positioning and role of metal ions in DNA recognition sites might reflect important properties of protein–DNA interaction. A...

Neisseria cuniculi produces the restriction enzyme NcuI which is an isoschizomer of MboII. We have demonstrated that NcuI recognizes a pentanucleotide sequence (5′-GAAGA-3′/3′-CTTCT-5′), and cleaves the DNA 8 and 7 nucleotides downstream from the recognition site leaving a single 3′-protrud...

The amino acid residue Asn141 of the restriction endonuclease EcoRI was proposed to make three hydrogen bonds to both adenine residues within the recognition sequence -GAATTC-. We have mutated Asn141 to alanine, aspartate, serine, and tyrosine. Only the serine mutant is active under normal buffe...

SummaryThe crystal structure of the rare-cutting HNH restriction endonuclease PacI in complex with its eight-base-pair target recognition sequence 5′-TTAATTAA-3′ has been determined to 1.9 Å resolution. The enzyme forms an extended homodimer, with each subunit containing two zinc-bound motifs ...

SummaryArchaeal NucS nuclease was thought to degrade the single-stranded region of branched DNA, which contains flapped and splayed DNA. However, recent findings indicated that EndoMS, the orthologous enzyme of NucS, specifically cleaves double-stranded DNA (dsDNA) containing mismatched bases. I...

Homing endonucleases are a group of site-specific endonucleases that initiate homing, a nonreciprocal transfer of its own gene into a new allele lacking this gene. This work describes a novel phage T4 endonuclease, SegD, which is homologous to the GIY-YIG family of homing endonucleases. Like oth...

Two synthetic procedures to enable a straightforward and efficient solid-phase synthesis of 3-aryl-3-oxo-propan amides (β-keto amides) are described and compared. Lithium enolates, which can be obtained by deprotonation of methyl ketones with LiHMDS, are added to either an immobilized isocyanat...

Toxicological and epidemiological studies have suggested the involvement of di-(2-ethylhexyl) phthalate (DEHP) in increases in allergies. The effects of DEHP have been implicated in the deflection to T helper 2 (Th2)-biased immune responses. However, we could not observe an adjuvant effect of DE...

Laser paint removal was studied with ns-pulsed fiber laser on the combination of 20 μm-thick, white polymeric paint and Al alloy substrate. The response of paint to single pulse ablation was evaluated to measure the ablated zone dimensions. With this information, the effect of overlap, number o...

Well crystallized and homogeneous iron doped LiCoPO4 (LCfP) thin films have been grown by ns Pulsed Laser Ablation, at ambient temperature without any substrate heating or post-annealing treatments. The films have been deposited in vacuum and in the presence of buffer gases (O2, Ar) and it has b...

We demonstrate a systematic and rational approach to create a library of natural and modified, dialkylated amino acids based upon arginine for development of an efficient small interfering RNA (siRNA) delivery system. These amino acids, designated DiLA2 compounds, in conjunction with other compo...

The binding of cationic peptides of the sequence (KX)4K to lipid vesicles of negatively charged dipalmitoyl-phosphatidylglycerol (DPPG) was investigated by differential scanning calorimetry (DSC) and temperature dependent Fourier-transformed infrared (FT-IR) spectroscopy. The hydrophobicity of t...

The absorption and circular dichroism (c.d.) spectra of poly(l-ornithine) (PLO) and poly(l-α,γ-diaminobutyric acid) (PLDA) azo dye complexes were measured. Two dichroic bands associated with the blue shifted absorption bands of methyl orange (MO) at around 370 nm were observed by complex forma...