The depolarizing activities of homocholine, homocholinethiol, homocholineselenol, their acetyl esters, and their methyl derivatives were measured in the single-cell electroplax preparation and compared with the activities of the corresponding series ofcholine, cholinethiol, and cholineselenol de...

Minces of mouse forebrain were incubated in Krebs or a Krebs solution containing high K+ (32.7mM) and Li+, instead of Na+, for 30 min at 37°; subcellular fractions were prepared, and the levels of ACh in the S3 and P3 fractions were determined and compared for the two treatments. Incubation of ...

Microbial transformation of homocholine plays a central role in many biological systems and influence on all kingdoms of life. Here, we used suppression subtractive hybridization (SSH) approach to screen for genes that differentially expressed in response to homocholine by Pseudomonas sp. strain...

Homocholine, a quaternary ammonium compound that resembles choline in many cholinergic metabolisms, is recently incorporated in many synthetic pharmaceuticals drugs targeting various types of cancer and bone marrow cells. Here, we used a proteomic approach (two-dimensional polyacrylamide gel ele...

An intracellular β-xylosidase (AbXyl), from the thermoalkaline Anoxybacillus sp. 3M, was purified and characterized. The homodimeric enzyme (140 kDa) was optimally active at 65 °C and pH 5.5, exhibited half life of 10 h at 60 °C, 78 and 88% residual activity after 24 h, at pH 4.5 and 8.0, res...

Currently, hyperstable endo-1,4-β-xylanase has been the focus of attention as potent biocatalyst as well as utilization in bioconversion process. Therefore, the gene (1035 bp) of a monomeric glycoside hydrolase family 10 (GH10) endo-1,4-β-xylanase (TnXynB) from a hyperthermophilic eubacterium ...

This work evaluated the suitability of various mesoporous silicas as carriers for developing an oral formulation endowed with improved dissolution properties of glibenclamide, hypoglycemic agent poorly water-soluble. The different silicas were examined for solid-state, morphology, and technologi...

Glibenclamide is an anti-hyperglycaemic drug that is commonly used for the treatment of type 2 diabetes mellitus and has promising new medical indications. However, this drug is associated with high rates of serious hypoglycaemic episodes as a result of its pharmacological activity. Administerin...

The commonly used sedative α2-adrenoceptor agonist dexmedetomidine has adverse cardiovascular effects in dogs that can be prevented by concomitant administration of the peripherally acting α2-adrenoceptor antagonist MK-467. An ancillary effect of dexmedetomidine is to decrease insulin release ...

Peceol containing niosomes (peceosomes) have been shown to widen absorption window of acidic drugs. However, the relationship between drug release and intestinal absorption is not clear. Accordingly, the objective was to probe peceosomes for enhancing intestinal absorption of glibenclamide with ...

In the present study, Glibenclamide (GLI) was determined in blood samples through the application of a method based on coupling low-voltage electromembrane extraction (LV-EME) and fast Fourier transform square wave voltammetry (FFTSWV). The parameters influencing the LV-EME of GLI (i.e. extracti...

The sulfonylurea receptor 1 (SUR1)-regulated NCca-ATP channels were progressively upregulated and demonstrated unchecked opening in central nervous system (CNS) injury, which induced cerebral damage. Glibenclamide (GLI) can block NCca-ATP channels and consequently exert protective effects. Recen...

Proteinuria is a common adverse effect of renal injury in preeclampsia. To explore the effects of AKR1C3 in renal injury due to preeclampsia and to determine an optimal method to prevent proteinuria, glibenclamide treatment was used in unrestrained Wistar rats exposed to N-nitro-L-arginine methy...

This research introduces a new modification of carbon paste electrode (CPE) by ZnIn2S4 nanoparticles (ZIS) and an ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate, (BMIM.PF6)) as a sensitive sensor for voltammetric detection of gliclazide and glibenclamide compared to unmodified car...

The uniform thin films with variable thicknesses (d = 49, 120, 220 nm) of active pharmaceutical ingredient (API) glibenclamide (GCM) was spin-coated and investigated using broadband dielectric, grazing incident FTIR spectroscopies, atomic force microscopy, and ellipsometry. Data analysis reveale...

Polyesters of (R)-3-hydroxybutanoic and (R)-3-hydroxypentanoic acids have been isolated from Aphanothece species, a freshwater cyanobacteri

Cu-Ni/SiO2 catalyst was developed by impregnation assisted with citric acid for the vapor-phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL). The prepared catalysts were characterized by using X-ray powder diffraction (XRD), transmission electron microscope (TEM), temperature-p...

A simple and reliable high performance liquid chromatographic (HPLC) method has been developed and validated to quantify cinepazide maleate, a calcium blocker, in rat plasma. Cinepazide maleate and Tinidazole (internal standard) have been extracted by a simple liquid–liquid extraction before in...

The crystal structure of potassium hydrogen maleate KH(OOC–CHCH–COO) has been studied at 300, 14 and 5 K using three-dimensional single-crystal neutron diffraction data. There is no evidence for any phase transition. The crystal structure is orthorhombic, Pbcm (D2hll), with four entities in ...

The spectral profiles of the hydrogen bond vibrations in the potassium hydrogen maleate (KHM) crystal and its deuterated analogue (KDM) were studied theoretically taking into account the Fermi resonance (FR) effect and strong coupling between the hydrogen bond vibration and lattice phonons. In a...