Within the past year, it has become apparent, in connection with its use on automatic flow cytometers, that the quality of commercially available Alcian Blue has significantly declined. A homologous series of alkylated (C1–C7) Astra Blue quaternary ammonium halides was prepared, characterized, ...

Dihydrofolate reductase was purified from mouse liver and spleen, and certain physical and kinetic properties were compared. The Chromatographic properties of the two enzymes on Sephadex G-75 were similar. Both enzymes exhibited two broad double pH optima, one between pH 4.5 and 5.5, and another...

5-Fluorouracil (5-FU) and its oral prodrug capecitabine are among the most widely used chemotherapeutics. For cytotoxic activity, 5-FU requires cellular uptake and intracellular metabolic activation. Three intracellular formed metabolites are responsible for the antineoplastic effect of 5-FU: 5-...

(Deoxy)guanosine-5′-triphosphate (5′-(d)GTP), the precursor for synthesizing DNA or RNA in vivo, is an important raw material for various modern biotechnologies based on PCR. In this study, we investigated the application of whole-cell catalysts constructed by bacterial cell surface display in...

The carcinogenicity of N-methyl-N-nitrosourea (MNU) arises from its ability to methylate DNA. This occurs in an aqueous environment and therefore an appreciation of the mode of decomposition of MNU in water is essential to understanding the mechanism of DNA methylation and its base sequence depe...

8-Methyladenosine 3′-monophosphate dihydrate was synthezied and crystallized in the monoclinic space group P21 with the unit cell dimensions: a = 9.09592) Å, b = 16.750(3) Å, c = 5.405(2) Å and β = 97.61(3)°. The structure was determined by the application of the heavy atom method and refi...

Adducts were prepared by reacting styrene oxide with 2-deoxyguanosine 3′-monophosphate (dGMP). Four isomeric N-7-, two diastereomeric N2- and three isomeric O6-adduct were isolated and characterized. The adducts were used as substrates in the 32P-postlabeling reaction. No phosphorylation produc...

Deoxyguanosine 3′-monophosphate (dGMP) was alkylated at the 7-position by dimethyl sulfate, ethylene oxide and styrene oxide in aqueous media and glacial acetic acid, respectively, to yield reasonable quantities of the products, which were purified by HPLC. dGMP adducts are needed as standards ...

The 32P-postlabeling technique was used to investigate the efficiency of phosphorylation reaction by T4 polynucleotide kinase using seven synthetic adducted deoxyguanosine 3′-monophosphates. The adducts included cyclic N1,N2 derivatives and the C8 adduct of 4-aminobiphenyl. The adducted substra...

The effect of increased cellular concentrations of adenosine 3′,5′ monophosphate (cAMP) upon mutation frequency induced by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) was studied in V79 Chinese hamster lung cells. Incubation with either forskolin, which increased the accumulation of cAMP, or ...

The effect of increased cellular concentrations of adenosine 3′,5′ monophosphate (cAMP) upon mutation frequency induced by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) was studied in V79 Chinese hamster lung cells. Incubation with either forskolin, which increased the accumulation of cAMP, or ...

Advances in the gas chromatography procedure for the identification of amino acid thiohydantoins include: (1) a new blend of silicone stationary phases (CFC), which provides superior resolving power than hitherto obtained with single phases or earlier blends; (2) use of a carrier gas helium, whi...

1. Modification of Escherichia coli tRNA with cyanogen bromide caused a selective loss of acceptor activities for glutamic acid, lysine and glutamine. However, most tRNA's known to contain 4-thiouridine, which is susceptible to cyanogen bromide, retained their amino acid acceptor activities...

Transfer RNAs from liver and brain of young and old BC3F1 mice were compared in regard to extent of aminoacylation and cochromatographic profiles of isoaccepting tRNA species on reversed-phase columns. Homologous synthetase preparations and optimal aminoacylation conditions were employed, having...

Media conditioned by compound 4880-stimulated rat mast cells generated immunoreactive histamine-releasing peptide (HRP) when incubated at physiological pH with bovine serum albumin and the carboxypeptidase inhibitor, O-phenanthroline. The generation of immunoreactive HRP (IR-HRP) was time (after...

Seven novel peptides were isolated from the skin secretions of the North American dusky gopher frog, Rana sevosa, on the basis of antimicrobial activity and histamine release from rat peritoneal mast cells. The peptides were purified to homogeneity using HPLC and characterized by electrospray io...

Human atrial natriuretic peptide [ANF(1–28)] contains five arginine residues and carries an overall positive change of four. It was hypothesized that atrial peptides may induce mast cell histamine release. In vitro, three atrial peptides [ANF(1–28), (3–28) and (5–28)] were demonstrated to in...

BackgroundApelin is a peptide that was originally isolated from bovine stomach extract and has been demonstrated to be an endogenous ligand for orphan receptor APJ. Both apelin and the APJ receptor are widely distributed in the whole body. Apelin is supposed to have important regulatory roles in...

We have been interested in the effects of some popular peptides on tracheal smooth muscle. Previously, we reported that atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) had dose-dependent relaxant effects on guinea pig tracheal smooth muscle....

We studied the effect of activating histamine H3 receptors (H3Rs) on rat nucleus accumbens (rNAcc) dopaminergic transmission by analyzing [3H]-dopamine uptake by synaptosomes, and dopamine synthesis and depolarization-evoked [3H]-dopamine release in slices. The uptake of [3H]-dopamine by rNAcc s...