Oligomycin resistant yeast mutants induced by ultraviolet irradiation were also resistant to rutamycin and/or venturicidin. One of the two mutants analyzed in detail showed cytoplasmic inheritance of oligomycin and rutamycin resistance while in the other strain, inheritance of oligomycin and ven...

The asymmetric synthesis of the spiroketal fragment of rutamycin B is reported employing allylsilane bond construction methodology for the introduction of five of the eight stereogenic centers. In this paper, the construction of the C19C28 and C29C34 fragments as well as their coupling throu...

The asymmetric synthesis of the polypropionate fragment of rutamycin B is reported employing chiral allylsilane bond construction methodology for the introduction of six of the nine stereogenic centers. In this paper, the construction of the C3C12 subunit and its coupling to the aldehyde 12 th...

A convergent synthesis of the spiroketal subunit of rutamycins A and B has been devised via Julia coupling of sulfone 14 with aldehyde 23; the pentahydroxy ketone 3 derived from 25 underwent spontaneous cyclization to spiroketal 4.

the asymmetric synthesis of the polypropionate segment of rutamycin B is reported. In this convergent synthesis the construction of the C1-C8 and C9-C17 subunits and their union through a double stereodifferentiating aldol fragment coupling are described. The stereoselectivity of this coupling r...

The applicability of Dextran Sulphate (DS) agarose in the purification of the VIII/vWf complex from cryoprecipitate was investigated. The elution profiles indicate high affinity of the VIII/vWf complex for the matrix. With application of combined salt/pH gradients good resolution of VIII/vWf fro...

A relatively simple and reproducible chromatographic separation using Dextran Sulphate (DS) Agarose is described for the purification of vWf:Ag from cryoprecipitate or plasma source material. The elution profiles suggest high affinity of vWf for the matrix permitting resolution from Fibrinogen, ...

Ristocetin A is one of a series of structurally related amphoteric, glycopeptide, macrocyclic antibiotics. These compounds have several features that make them attractive as chiral selectors. These include spatially oriented functional groups that are known to provide the types of interactions t...

The antibiotic ristocetin is a glycoproteoid isolated from the culture media of Nocardia lurida N.R.R.L. 2430 whose structural features have yet to be resolved (1–3). The antibiotic has been studied both for its antibiotic properties (4–6) and, more recently, for its inducement of platelet agg...

Selectively and totally sulfated (1 → 3)-linked linear homofucans bearing ∼20 monosaccharide residues on average have been prepared from the branched xylofucan sulfate isolated from the brown alga Punctaria plantaginea. Anticoagulant and antithrombotic properties of the parent biopolymer and i...

Polymyxin B is one of the last resort option for carbapenem-resistant Klebsiella pneumoniae (CRKP) bloodstream infection in China. Therefore, the timing of administration of polymyxin is frequently delayed. We collected 40 cases of CRKP bloodstream infections (BSIs) treated with combinations bas...

Little is known regarding the appropriate timing and sequencing of a carbapenem and polymyxin in combination against carbapenem-resistant Enterobacteriaceae. Meropenem and polymyxin B were administered simultaneously or 1 agent 2 h prior to the other, in vitro. The carbapenem should be administe...

Here, we evaluated the combinations of antibiotics polymyxin B (PMB), tigecycline (TGC) and meropenem (MEM) by time-kill curves (TKC) against carbapenem-resistant Enterobacter cloacae isolates. Combination of PMB/TGC and PMB/MEM showed promising results in sub-inhibitory concentration of PMB ind...

ABSTRACTKlebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-KP) bloodstream infections (BSIs) are related to high mortality rates, and combination therapy has been associated with lower mortality in patients treated mostly with colistin. There is a paucity of studies addressing poly...

Antimicrobial resistance is increasing and few new antibiotics are in the development pipeline. Alternative strategies to treat infectious diseases, such as combination therapy, are urgently needed. Polymyxin B is a neglected and disused antibiotic with moderate antibacterial activity. In this s...

ObjectivesPolymyxins have been revitalized to combat carbapenem-resistant Enterobacteriaceae (CRE). However, evaluating the activity of these agents by traditional broth dilution methods is not practical for busy clinical laboratories. We compared polymyxin B activity using two quantitative susc...

ABSTRACTA number of companies manufacture polymyxin B using United States Pharmacopeia (USP) metrics, rather than chemical composition, to report biological activity. Given that polymyxin B contains several different components, it is unknown whether pharmacokinetic and pharmacodynamic variabili...

ObjectivesThe objective of this study was to describe the preparation and in vitro evaluation of the surface modified nanostructured lipid carrier (NLC) using chitosan and dextran in the co-delivery of buparvaquone (BPQ) and polymyxin B (PB) against leishmaniasis.

Infections caused by Acinetobacter baumannii are difficult to treat as they are often multidrug resistant (MDR) and frequently form biofilms. We investigated the activities of minocycline, polymyxin B, meropenem, and amikacin against diverse Acinetobacter baumannii strains with biofilm formation...

Polymyxin B (PMB) and polymyxin E (PME) are cyclic, peptide antibiotics which derived from various species of Paenibacillus (Bacillus) polymyxa. They are decapeptide antibiotics with an antimicrobial spectrum that includes Gram-negative bacteria, and reused as therapeutic agents due to the emerg...