The effect on the oral microbial flora of lozenges containing Actamer® and tyrothricin, singly and in combination, has been determined. The agents when administered alone significantly decreased the total numbers of microorganisms for at least one- half hour after dissolution of a single lozenge...

Eight patients with tuberculous empyema were treated with tyrothricin in amounts varying from 10 to 50 mg. The temperature was elevated following the instillation of this drug in all cases. In three cases chest pain was experienced and one patient complained of dyspnea eight hours after the inst...

A rapid, sensitive, and accurate reversed-phase HPLC method has been developed for the analysis and quantification of pharmaceutical formulations containing tyrothricin (1), an antibiotic used in antiseptic buccal compressed tablets for local application. The assay has been carried out under iso...

Tandem mass spectrometry has been shown to be a powerful technique for determining the structures of biological compounds. This paper details the mass spectrometric methods employed to characterise the structural variations found within a mixture of cyclic decapeptides, tyrothricin, produced by ...

Methods are reported for the determination of tyrothricin and benzocaine by HPLC and menthol by GC in the analysis of throat lozenges (tablets) containing all three compounds. After optimization of the variables involved in both HPLC and GC the methods have been characterized and validated accor...

Kinetic methodology was used for the first time to develop a simple, fast spectrofluorimetric method for the determination of neomycin and tyrothricin using the stopped-flow mixing technique. The proposed method involves reaction of these antimicrobial substances with o-phthaldialdehyde in the p...

Background: Many patients with chronic dermatophytosis and onychomycosis have depressed cell-mediated immunity (CMI) to trichophytin. Objective: The fungicidal properties of oral terbinafine provide a unique opportunity to explore whether elimination of antigen could restore CMI response in thes...

No information is available on the structural requirements for the antitumor activity of sparsomycin, an antibiotic obtained from the fermentation broth of Streptomyces sparsogenes. Its high in vivo and in vitro activity, novel structure, and uncommon mode of action have, therefore, suggested th...

Three pyrimidinylpropanamide antibiotics sparsomycin (1), sparoxomycins A1, A2 (2, 3), and also six analogues (4–9) have been synthesized by employing asymmetric sulfide oxidation conditions as a key step. Sparsomycin (1) and its alkyl analogues (5–7) showed higher morphological reversion acti...

Precursor incorporation experiments have ruled out the involvement of N′-formylkynurenine in the biosynthesis of the uracil acrylic acid moiety of sparsomycin. An enzyme has been partially purified from the sparsomycin-producer that catalyzes the synthesis of the uracil acrylic acid from a pyri...

Ten analogues of the antibiotic sparsomycin were prepared and evaluated in several in vitro tests. Nine of them carry a modification at the hydroxymethylene group of the molecules, two have a disulfide bond instead of the S(O)-CH2-S moiety at the sulfur-containing side chain of the molecules. Wh...

Sparsomycin (Sm) is a known antibiotic derived from Streptomyces. Its potential antitumor activity stimulated the search for a synthetic production method and the development of new derivatives. In a recent screening investigation, three Sm analogues appeared to be more active and considerably l...

The colony formation in agar of human tumour xenografts was used as a test system to study the cytostatic activity of ethyldeshydroxy-sparsomycin (EdSm) at the cellular level. EdSm was additionally studied in vivo in human rumour xenografts and murine tumour models. EdSm showed a clear dose-resp...

Hundred and twenty microbial isolates could be isolated from different soil samples collected from different localities in Egypt. One of the bacterial cultures AZ-SH-B8 was found to produce a wide spectrum antimicrobial agent when cultivated on plastic wastes. The bacterial culture AZ-SH-B8 coul...

New insights into the way that the tRNA:mRNA complex is translocated on the ribosome during protein synthesis have come from the recent observation that binding of the antibiotic sparsomycin in the peptidyl transferase center can trigger these large-scale movements.

ObjectivesSparsomycin, which exhibits rare broad-spectrum antibiotic and antitumour activity against bacteria, Archaea, Eucarya and various cancer cell lines, has been widely used as a powerful tool to study protein synthesis. Here we report the draft genome sequence of Streptomyces sparsogenes ...

Serial dilutions of mixtures of the closely related diterpenoid orthoesters simplexin and huratoxin were added to cultures of chicken embryo fibroblast cells and bovine erythroid cells; the mixture was lethal at concentrations of 100 μg/ml and 1 μg/ml, respectively. Morphological changes were ...

The diastereofacial selectivities of aldehyde 2b and ketone 3 are metal dependent, and their fragment assembly aldol coupling is a matched double asymmetric reaction when a chlorotitanium enolate is used.

1.(1) F0·F1ATPase (EC 3.6.1.3) from Micrococcus luteus ATCC 4698 was solubilized from plasma membranes by the non-ionic detergent Triton X-100 in the presence of 0.05 M MgCl2.2.(2) The antibiotics rutamycin, Dio-9, quercetin, oligomycin, botrycidin, efrapeptin, leucinostatin, valinomycin, and v...

Publisher SummaryThe assay used for the purification of reconstitutively active rutamycin-sensitive ATPase (RSA) preparation is the 32Pi–ATP exchange. The most convenient procedure for the reconstitution of vesicles catalyzing the exchange is the cholate dilution method. Soybean phospholipids (...