The kinetic behavior of a thrombin-like enzyme from Lachesis muta muta venom has been studied with 13 tripeptidyl p-nitroanilide substrates. Eight substrates were unprotected at the N terminus and were used for the regression analysis of the experimentally determined kinetic parameters 1/Km, kca...
The steady-state kinetic parameters of the tripeptides d-Val-Leu-Lys-, Ala-Phe-Lys-, and
The hydrolysis of tripeptidyl-p-nitroanilides by human plasmin /EC 3.4.21.7./ was studied and the kinetic parameters were determined. The individual contributions of the amino acid side chains at the P1-P4 subsites to the kinetic parameters were calculated by regression analysis. The highest con...
This letter describes the parallel synthesis of dipeptide p-nitroanilides (1) and dipeptide diphenyl phosphonates (2), compounds that can be used as substrates and irreversible inhibitors for the rapid profiling of dipeptidyl peptidases. A polymer-assisted solution-phase synthesis was used for a...
The steady-state kinetics of plasmin- (EC 3.4.21.7) and trypsin-catalysed (EC 3.4.21.4) hydrolysis of Bz-l-Phe-Val-Arg-pNA, Bz-d-Phe-Val-Arg-pNA, l-Phe-Val-Arg-pNA, d-Phe-Val-Arg-pNA and d-Val-Leu-Lys-pNA were investigated in the pH range 6–9. The pH dependences of the kinetic parameters corres...
In mice, the initial stage of nephrotoxic serum-induced nephritis (NTN) mimics antibody-mediated human glomerulonephritis. Local immune deposits generate tumor necrosis factor (TNF), which activates pro-inflammatory pathways in glomerular endothelial cells (GECs) and podocytes. Because TNF recep...
Bee pollen (BP) is a natural medicine from the hive with various potential health-promoting benefits, but until now there is no study to determine its protective roles in inflammatory bowel disease (IBD). The aim of this study was to reveal the in vitro gastrointestinal protective effects of BP ...
Considering the vital role of skeletal muscle in control of whole-body metabolism and the severity of long-term diabetic complications, we aimed to reveal the molecular pattern of early diabetes-related skeletal muscle phenotype in terms of energy metabolism, focusing on regulatory mechanisms, a...
ObjectiveConsidering the diverse mechanisms by which fluoride could affect oral bacteria, this study evaluated the effect of sodium fluoride onStreptococcus mutans transcriptome in the presence of sucrose.
The phenylthiohydantoin (PTH) derivatives of the common amino acids can be resolved in a single high-performance liquid chromatographic analysis by elution from a cyanopropylsilane column with a ternary gradient of methanol, acetonitrile, and ammonium acetate. The system is compatible with an au...
A bonded tripeptide (l-valyl-l-phenylalanyl-l-valine) phase has been used as a stationary phase in liquid chromatography. Unique retention orders and retention variations are shown for phenylthiohydantoin (PTH) derivatives of amino acids and isomeric dipeptides using acidic and basic mobile phas...
A high-performance liquid chromatography system is presented which allows separation and quantitation (in the range 4–1000 pmol) of all common phenylthiohydantoin amino acids, including derivatives of 4-hydroxyproline, methionine sulfone and three differently modified forms of cysteine. By show...
Publisher SummaryAmino acid analysis provides an important quantitative parameter in the characterization of isolated protein or peptide samples. Because of the availability of highly sensitive instruments for determining amino acids in the picomole range, the preparation of the sample for analy...
Phenylthiohydantoin (PTH) amino acids, the derivatives of amino acids liberated in the course of automated N-terminal sequence analysis of peptides and proteins, are most commonly identified by high-performance liquid chromatography. This communication describes an extension to the methodology f...
Advances in liquid chromatography have brought about the development of new techniques in amino acid analysis which take full advantage of precolumn derivatization procedures. Using phenylisothiocyanate as the reagent, detection limits under 1 pmol can be routinely achieved, allowing the analysi...
We have isolated a novel acidic amino-acid-specific proteinase from Streptomyces fradiae ATCC 14544, using benzyloxycarbonyl-l-Phe-l-Leu-l-Glu-p-nitroanilide (Z-Phe-Leu-Glu-pNA) as a substrate. A proteinase, which we propose to call SFase, was purified from the culture filtrate by salting out, r...
Thin-layer chromatography (TLC) is a simple and inexpensive technique permitting a number of samples to be handled simultaneously, thus yielding a higher precision than sequential analysis. The inert character of the thin-layer material makes it ideally suitable for use with strong corrosive rea...
Publisher SummaryThe saturated hydroxydicarboxylic acids have the general formula CnH2n-1 (OH) (CO2H)2, and its two types may be distinguished as: hydroxyl-substituted acids of the series HO2C(CH2)nCO2H, for example, hydroxymalonic acid and hydroxysuccinic acid together with their alkyl-substitu...
Publisher SummaryAliphatic monocarboxylic acids containing an additional carbonyl group in the carbon chain are known generically as oxo-carboxylic acids. They are conveniently divided into two main classes depending on the position of the carbonyl group. When this occupies a terminal position, ...
Excitatory amino acids, such as N-methyl-D aspartate (NMDA) and kainate, promote neuritogenesis and viability in primary cultures of cerebellar granule cells. In view of the recent demonstration that excitatory amino acids activate the synthesis of nitric oxide, the present study examined a pote...
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