The aim of the paper was the identification and the quantitative evaluation of the following inorganic anions: chloride, phosphate, nitrate, nitrite, sulphate and the following organic acids: lactic, acetic, formic, malic and citric in commercial “unrefined” brown cane sugars and in cane raw s...
SummaryEndoplasmic reticulum, Golgi apparatus, and transit vesicles, the organelles involved in intracellular transport of storage proteins, were isolated from developing pea cotyledons. After organelle lysis and washing of the membranes with salt to remove the bulk of the matrix proteins, addit...
This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy).This article has been retracted at the request of the Editor. Data included in Figure 6 had been published previously by the same authors in the Journal of Molecula...
A metabolic pathway for high level production of N-acetylglucosamine has been engineered in Escherichia coli by overexpressing E. coli glucosamine synthase (GlmS) and Saccharomyces cerevisiae glucosamine-6-phosphate acetyltransferase (GNA1). GlmS catalyzes the synthesis of glucosamine-6-phosphat...
We report the crystal structure of the apoenzyme of N-acetylglucosamine-6-phosphate (GlcNAc6P) deacetylase from Escherichia coli (EcNAGPase) and the spectrometric evidence of the presence of Zn2+ in the native protein. The GlcNAc6P deacetylase is an enzyme of the amino sugar catabolic pathway th...
The use of pentenyl and thiophenyl glycosides of N-acetylglucosamine (GlcNAc) as glycosyl donors for the direct preparation of O-glycosides of GlcNAc promoted by N-iodosuccinimide (NIS) and metal triflates in dichloromethane has been investigated. Both glycosyl acceptors 1-octanol and (−)-mentho...
Cells of Micrococcus sp. 2102 incorporate inorganic [32P]phosphate from the medium into the sugar-phosphate polymer of the wall. Controlled acid hydrolysis of sodium dodecyl sulphate-extracted cells gives N-acetylglucosamine 6-[32P]phosphate which can be purified by ion-exchange chromatography a...
N-Acetylglucosamine (O-GlcNAc) modification on serine or threonine residues of cytoplasmic and nuclear proteins has become a more recognized intracellular covalent modification. Removal of this modification is carried out by N-acetyl-β-glucosaminidase (O-GlcNAcase). Since little information exi...
Glucosamine and N-acetylglucosamine are currently produced by extraction and acid hydrolysis of chitin from shellfish waste. Production could be limited by the amount of raw material available and the product potentially carries the risk of shellfish protein contamination. Escherichia coli was m...
(d)-Glucosamine and other nutritional supplements have emerged as safe alternative therapies for osteoarthritis, a chronic and degenerative articular joint disease. N-acetyl-(d)-glucosamine, a compound that can be modified at the N position, is considered to improve the oral bioavailability of (...
The important platform polysaccharide N-acetylglucosamine (GlcNAc) has great potential to be used in the fields of food, cosmetics, agricultural, pharmaceutical, medicine and biotechnology. This GlcNAc is being produced by traditional methods of environment-unfriendly chemical digestion with str...
A charged and size uniform polysaccharide PS2 was prepared from red seaweed Polysiphonia senticulosa Harvey. Based on nuclear magnetic resonance (NMR), fourier transform infrared (FTIR) spectroscopy, and electrospray ionization collision-induced-dissociation mass spectrometry (ESI-CID-MSn) analy...
Evidence is presented demonstrating the ability of Ralstonia eutropha A5 to degrade 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD) aerobically. Strain A5 was able to effect significant transformation of [14C]DDD: the hexane extractable radioactivity decreased to approximately 50% of the contro...
The macromolecular binding of 4,4′-methylene-bis(2-chloroaniline) (MOCA), a suspect human carcinogen, was studied in the adult male Sprague-Dawley rat after both oral and dermal administration. Rats were euthanized 1, 3, 7, 10, 14, and 29 days after a single 281 μmol/kg body wt dose of [14C]MO...
The preparation of 14C-labelled biphenyl, 2,5-dichlorobiphenyl, 2,4′,5-trichlorobiphenyl, 2,2′,4,5′-tetrachlorobiphenyl, 2′,3,4,4′,5-pentachlorobiphenyl, 2,2′,3,4,4′-pentachlorobiphenyl, 2,3,3′,4′,6-pentachlorobiphenyl and 2,2′,3,3′,6-pentachlorobiphenyl is described [14C]Aniline hydr...
The effect of multiple oral administration of MOCA, a suspect human carcinogen, was studied in the adult male rat. As many as 28 consecutive daily doses of [14C]MOCA at 28.1 μmol/kg body wt (5 μCi/day) were administered and rats were euthanized at weekly intervals for 7 weeks. MOCA adduct form...
Extensive research has been conducted over the past decades to develop alternatives to the rabbit eye irritation test (Draize test) used in a regulatory context to assess eye irritation potentials. Although no single in vitro test has emerged as being completely acceptable for full replacement, ...
An extracellular pectate lyase (EC 4.2.2.2) was purified from the culture filtrate of a newly isolated Bacillus pumilus DKS1 grown in pectin containing medium. Using ion-exchange and gel filtration chromatography, this enzyme was purified and found to have a molecular weight of around 35 kDa. Th...
The interaction of divalent cations with potassium pectate and three potassium pectinate samples with degrees of esterification E of 23.8, 59.1 and 93.4% were studied by FT-IR spectroscopy. Characteristic shifts occurred in C–O and ring vibrations in the 1200–900 cm−1 region as well as in the ...
Uridine phosphorylase (UP) is a key enzyme in the pyrimidine salvage pathway, catalyzing the reversible phosphorolysis of uridine to uracil and ribose-1-phosphate (R1P). The human UP type 1 (hUP1) is a molecular target for the design of inhibitors intended to boost endogenous uridine levels to r...
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